product summary
Loading...
company name :
Synaptic Systems
product type :
antibody
product name :
MLC-2A
catalog :
311 011
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
56F5
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:200; fig 3a
Lalit P, Rodriguez A, Downs K, Kamp T. Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos. Nat Protoc. 2017;12:1029-1054 pubmed publisher
  • flow cytometry; human; 1:100; fig s1
Mannhardt I, Breckwoldt K, Letuffe Brenière D, Schaaf S, Schulz H, Neuber C, et al. Human Engineered Heart Tissue: Analysis of Contractile Force. Stem Cell Reports. 2016;7:29-42 pubmed publisher
  • flow cytometry; human; fig s1
Maillet A, TAN K, Chai X, Sadananda S, Mehta A, Ooi J, et al. Modeling Doxorubicin-Induced Cardiotoxicity in Human Pluripotent Stem Cell Derived-Cardiomyocytes. Sci Rep. 2016;6:25333 pubmed publisher
  • immunocytochemistry; mouse; fig s2
Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, et al. Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions. Stem Cell Reports. 2015;5:1128-1142 pubmed publisher
  • immunocytochemistry; mouse
Fu Y, Huang C, Xu X, Gu H, Ye Y, Jiang C, et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails. Cell Res. 2015;25:1013-24 pubmed publisher
Atmanli A, Hu D, Deiman F, van de Vrugt A, Cherbonneau F, Black L, et al. Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity. elife. 2019;8: pubmed publisher
Cao Y, Xu J, Wen J, Ma X, Liu F, Li Y, et al. Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules. Cell Physiol Biochem. 2018;50:538-551 pubmed publisher
Krause J, Löser A, Lemoine M, Christ T, Scherschel K, Meyer C, et al. Rat atrial engineered heart tissue: a new in vitro model to study atrial biology. Basic Res Cardiol. 2018;113:41 pubmed publisher
Ting S, Lam A, Tong G, Chen A, Wei H, Wu J, et al. Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess. Stem Cell Res. 2018;31:161-173 pubmed publisher
Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, et al. Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells. Tissue Eng Part C Methods. 2018;24:56-67 pubmed publisher
Tan Y, Han P, Gu Q, Chen G, Wang L, Ma R, et al. Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions. J Tissue Eng Regen Med. 2018;12:153-163 pubmed publisher
Vestergaard M, Grubb S, Koefoed K, Anderson Jenkins Z, Grunnet Lauridsen K, Calloe K, et al. Human Embryonic Stem Cell-Derived Cardiomyocytes Self-Arrange with Areas of Different Subtypes During Differentiation. Stem Cells Dev. 2017;26:1566-1577 pubmed publisher
Maillet A, Tan K, Brunham L. Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity. Curr Protoc Toxicol. 2017;73:22.5.1-22.5.22 pubmed publisher
Lin Y, Linask K, Mallon B, Johnson K, Klein M, Beers J, et al. Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med. 2017;6:527-538 pubmed publisher
Pei F, Jiang J, Bai S, Cao H, Tian L, Zhao Y, et al. Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells. Stem Cell Res. 2017;19:94-103 pubmed publisher
Wiencierz A, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, et al. Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes. PLoS ONE. 2015;10:e0143538 pubmed publisher
Fanton Y, Robic B, Rummens J, Daniëls A, Windmolders S, Willems L, et al. Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI. Int J Cardiol. 2015;201:10-9 pubmed publisher
Dorn T, Goedel A, Lam J, Haas J, Tian Q, Herrmann F, et al. Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity. Stem Cells. 2015;33:1113-29 pubmed publisher
Mehta A, Ramachandra C, Sequiera G, Sudibyo Y, Nandihalli M, Yong P, et al. Phasic modulation of Wnt signaling enhances cardiac differentiation in human pluripotent stem cells by recapitulating developmental ontogeny. Biochim Biophys Acta. 2014;1843:2394-402 pubmed publisher
Burridge P, Matsa E, Shukla P, Lin Z, Churko J, Ebert A, et al. Chemically defined generation of human cardiomyocytes. Nat Methods. 2014;11:855-60 pubmed publisher
Iglesias García O, Pelacho B, Prósper F. Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling. J Mol Cell Cardiol. 2013;62:43-50 pubmed publisher
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp T, et al. Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers. Circ Res. 2012;110:1556-63 pubmed publisher
Naaijkens B, Niessen H, Prins H, Krijnen P, Kokhuis T, De Jong N, et al. Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications. Cell Tissue Res. 2012;348:119-30 pubmed publisher
Jung C, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, et al. Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia. EMBO Mol Med. 2012;4:180-91 pubmed publisher
Herrmann F, Bundschu K, Kühl S, Kuhl M. Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos. Dev Dyn. 2011;240:2634-45 pubmed publisher
Elliott D, Braam S, Koutsis K, Ng E, Jenny R, Lagerqvist E, et al. NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods. 2011;8:1037-40 pubmed publisher
Smits A, Van Laake L, Den Ouden K, Schreurs C, Szuhai K, van Echteld C, et al. Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium. Cardiovasc Res. 2009;83:527-35 pubmed publisher
Van Laake L, Passier R, Monshouwer Kloots J, Verkleij A, Lips D, Freund C, et al. Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction. Stem Cell Res. 2007;1:9-24 pubmed publisher
Rust W, Balakrishnan T, Zweigerdt R. Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression. Regen Med. 2009;4:225-37 pubmed publisher
Zhang J, Wilson G, Soerens A, Koonce C, Yu J, Palecek S, et al. Functional cardiomyocytes derived from human induced pluripotent stem cells. Circ Res. 2009;104:e30-41 pubmed publisher
Phillips B, Horne R, Lay T, Rust W, Teck T, Crook J. Attachment and growth of human embryonic stem cells on microcarriers. J Biotechnol. 2008;138:24-32 pubmed publisher
Xu X, Zweigerdt R, Soo S, Ngoh Z, Tham S, Wang S, et al. Highly enriched cardiomyocytes from human embryonic stem cells. Cytotherapy. 2008;10:376-89 pubmed publisher
Phillips B, Lim R, Tan T, Rust W, Crook J. Efficient expansion of clinical-grade human fibroblasts on microcarriers: cells suitable for ex vivo expansion of clinical-grade hESCs. J Biotechnol. 2008;134:79-87 pubmed publisher
Mummery C, van der Heyden M, De Boer T, Passier R, Ward D, van den Brink S, et al. Cardiomyocytes from human and mouse embryonic stem cells. Methods Mol Med. 2007;140:249-72 pubmed
Clements R, Sodha N, Feng J, Mieno S, Boodhwani M, Ramlawi B, et al. Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass. J Thorac Cardiovasc Surg. 2007;134:1461-70 pubmed
Dai W, Field L, Rubart M, Reuter S, Hale S, Zweigerdt R, et al. Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts. J Mol Cell Cardiol. 2007;43:504-16 pubmed
product information
Cat. Nr. :
311 011
Antigen :
MLC-2A
Synonyms :
Myosin
Host Species :
mouse
Tpye :
monoclonal
Form :
purified IgG
Cell Line :
56F5
Subtype :
IgG2b
Immunogen :
Recombinant protein corresponding to AA 1 to 175 from human MLC-2A (UniProt Id: Q01449)
Reactivity :
human, rat, mouse
WB :
WB
ICC :
ICC
IHC :
IHC
IHC P :
IHC-P
Quantity :
100 µg
more info or order :
company information
Synaptic Systems
Rudolf-Wissell-Str. 28
37079 Goettingen
lab@sysy.com
http://www.sysy.com
49-551-50556-0
headquarters: germany