product summary
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company name :
Synaptic Systems
product type :
antibody
product name :
Gephyrin
catalog :
147 111
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B11
reactivity :
human, mouse, rat, zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:5000; loading ...; fig 6d
Yoshida T, Yamagata A, Imai A, Kim J, Izumi H, Nakashima S, et al. Canonical versus non-canonical transsynaptic signaling of neuroligin 3 tunes development of sociality in mice. Nat Commun. 2021;12:1848 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2e
Han W, Li J, Pelkey K, Pandey S, Chen X, Wang Y, et al. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science. 2019;366:246-250 pubmed publisher
  • western blot; mouse; 1:1000; fig 3g
Chmielewska J, Kuzniewska B, Milek J, Urbanska K, Dziembowska M. Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage. Mol Neurobiol. 2019;56:2741-2759 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 4b
Pellegrini L, Hauser D, Li Y, Mamais A, Beilina A, Kumaran R, et al. Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet. 2018;27:3257-3271 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4e
  • western blot; mouse; 1:50; loading ...; fig 4a
Kumar A, Dejanovic B, Hetsch F, Semtner M, Fusca D, Arjune S, et al. S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency. J Clin Invest. 2017;127:4365-4378 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig s2e
  • western blot; mouse; 1:10,000; loading ...; fig 4c
Tanabe Y, Naito Y, Vasuta C, Lee A, Soumounou Y, Linhoff M, et al. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2?. Nat Commun. 2017;8:408 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Daniel J, Cooper B, Palvimo J, Zhang F, Brose N, Tirard M. Analysis of SUMO1-conjugation at synapses. elife. 2017;6: pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3-s1a
Ripamonti S, Ambrozkiewicz M, Guzzi F, Gravati M, Biella G, Bormuth I, et al. Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. elife. 2017;6: pubmed publisher
  • western blot; mouse; loading ...; fig s4b
Li J, Casteels T, Frogne T, Ingvorsen C, Honore C, Courtney M, et al. Artemisinins Target GABAA Receptor Signaling and Impair ? Cell Identity. Cell. 2017;168:86-100.e15 pubmed publisher
  • immunohistochemistry; rat; loading ...; fig s2
Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun. 2016;7:13365 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:250; loading ...; fig 4b
  • western blot; rat; 1:1000; loading ...; fig 1d
Gross G, Straub C, Perez Sanchez J, Dempsey W, Junge J, Roberts R, et al. An E3-ligase-based method for ablating inhibitory synapses. Nat Methods. 2016;13:673-8 pubmed publisher
  • immunohistochemistry; rat; loading ...; fig 5e
  • western blot; rat; loading ...; fig 2b
Um J, Choii G, Park D, Kim D, Jeon S, Kang H, et al. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation. J Biol Chem. 2016;291:10119-30 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6a
Valenza M, Chen J, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, et al. Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice. EMBO Mol Med. 2015;7:1547-64 pubmed publisher
  • immunocytochemistry; mouse; fig 7
Bender J, Engeholm M, Ederer M, Breu J, Møller T, Michalakis S, et al. Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif. PLoS ONE. 2015;10:e0136768 pubmed publisher
  • western blot; mouse; 1:3000
Chugh D, Ali I, Bakochi A, Bahonjic E, Etholm L, Ekdahl C. Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2. PLoS ONE. 2015;10:e0132366 pubmed publisher
  • western blot; human; fig 5
Antonelli R, Pizzarelli R, Pedroni A, Fritschy J, Del Sal G, Cherubini E, et al. Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction. Nat Commun. 2014;5:5066 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig e4
Karayannis T, Au E, Patel J, Kruglikov I, Markx S, Delorme R, et al. Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission. Nature. 2014;511:236-40 pubmed
Kim H, Yang Y, Hwang H, Lee H, Jang H, Kim J, et al. Deletion of PLCγ1 in GABAergic neurons increases seizure susceptibility in aged mice. Sci Rep. 2019;9:17761 pubmed publisher
Bessières B, Jia M, Travaglia A, Alberini C. Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat basolateral amygdala. Learn Mem. 2019;26:436-448 pubmed publisher
Sessa A, Fagnocchi L, Mastrototaro G, Massimino L, Zaghi M, Indrigo M, et al. SETD5 Regulates Chromatin Methylation State and Preserves Global Transcriptional Fidelity during Brain Development and Neuronal Wiring. Neuron. 2019;: pubmed publisher
Terreros Roncal J, Flor García M, Moreno Jiménez E, Pallas Bazarra N, Rabano A, Sah N, et al. Activity-dependent reconnection of adult-born dentate granule cells in a mouse model of frontotemporal dementia. J Neurosci. 2019;: pubmed publisher
Rhee H, Shaib A, Rehbach K, Lee C, Seif P, Thomas C, et al. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons. Cell Rep. 2019;27:2212-2228.e7 pubmed publisher
Petrov A, Lam M, Mast N, Moon J, Li Y, Maxfield E, et al. CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice. Neurotherapeutics. 2019;: pubmed publisher
Dejanovic B, Huntley M, De Mazière A, Meilandt W, Wu T, Srinivasan K, et al. Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies. Neuron. 2018;100:1322-1336.e7 pubmed publisher
Letellier M, Szíber Z, Chamma I, Saphy C, Papasideri I, Tessier B, et al. A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation. Nat Commun. 2018;9:3979 pubmed publisher
Jia M, Travaglia A, Pollonini G, Fedele G, Alberini C. Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex. Learn Mem. 2018;25:533-543 pubmed publisher
Trobiani L, Favaloro F, Di Castro M, Di Mattia M, Cariello M, Miranda E, et al. UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism. Neurobiol Dis. 2018;120:139-150 pubmed publisher
Lee S, Shin S, Zhong P, Kim H, Kim D, Kim J, et al. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nat Commun. 2018;9:3434 pubmed publisher
Früh S, Tyagarajan S, Campbell B, Bosshard G, Fritschy J. The catalytic function of the gephyrin-binding protein IQSEC3 regulates neurotransmitter-specific matching of pre- and post-synaptic structures in primary hippocampal cultures. J Neurochem. 2018;147:477-494 pubmed publisher
Koga K, Shimoyama S, Yamada A, Furukawa T, Nikaido Y, Furue H, et al. Chronic inflammatory pain induced GABAergic synaptic plasticity in the adult mouse anterior cingulate cortex. Mol Pain. 2018;14:1744806918783478 pubmed publisher
Li Y, Kim R, Cho Y, Song W, Kim D, Kim K, et al. Lrfn2-Mutant Mice Display Suppressed Synaptic Plasticity and Inhibitory Synapse Development and Abnormal Social Communication and Startle Response. J Neurosci. 2018;38:5872-5887 pubmed publisher
Mukherjee J, Cardarelli R, Cantaut Belarif Y, Deeb T, Srivastava D, Tyagarajan S, et al. Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses. Proc Natl Acad Sci U S A. 2017;114:11763-11768 pubmed publisher
de Groot C, Floriou Servou A, Tsai Y, Früh S, Kohler M, Parkin G, et al. RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of ?2 GABAA receptors. PLoS Genet. 2017;13:e1007073 pubmed publisher
Smith K, Jones K, Kopeikina K, Burette A, Copits B, Yoon S, et al. Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins. J Neurosci. 2017;37:11127-11139 pubmed publisher
Hoon M, Krishnamoorthy V, Gollisch T, Falkenburger B, Varoqueaux F. Loss of Neuroligin3 specifically downregulates retinal GABAA?2 receptors without abolishing direction selectivity. PLoS ONE. 2017;12:e0181011 pubmed publisher
Acuna C, Liu X, Sudhof T. How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs. Neuron. 2016;91:792-807 pubmed publisher
Walker K, Modgil A, Albrecht D, Lomoio S, Haydon P, Moss S, et al. Genetic Deletion of the Clathrin Adaptor GGA3 Reduces Anxiety and Alters GABAergic Transmission. PLoS ONE. 2016;11:e0155799 pubmed publisher
Ren Z, Pribiag H, Jefferson S, Shorey M, Fuchs T, Stellwagen D, et al. Bidirectional Homeostatic Regulation of a Depression-Related Brain State by Gamma-Aminobutyric Acidergic Deficits and Ketamine Treatment. Biol Psychiatry. 2016;80:457-468 pubmed publisher
Nakamura Y, Morrow D, Modgil A, Huyghe D, Deeb T, Lumb M, et al. Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged ?-Aminobutyric Acid Receptor, Type A (GABAA), ?2 Subunit Knock-in Mouse. J Biol Chem. 2016;291:12394-407 pubmed publisher
O Sullivan G, Jedlicka P, Chen H, Kalbouneh H, Ippolito A, Deller T, et al. Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice. Mol Cell Neurosci. 2016;72:101-13 pubmed publisher
Lenz M, Galanis C, Müller Dahlhaus F, Opitz A, Wierenga C, Szabó G, et al. Repetitive magnetic stimulation induces plasticity of inhibitory synapses. Nat Commun. 2016;7:10020 pubmed publisher
Dejanovic B, Djémié T, Grünewald N, Suls A, Kress V, Hetsch F, et al. Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy. EMBO Mol Med. 2015;7:1580-94 pubmed publisher
Jedlicka P, Vnencak M, Krueger D, Jungenitz T, Brose N, Schwarzacher S. Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo. Brain Struct Funct. 2015;220:47-58 pubmed
Papadopoulos T, Schemm R, Grubmüller H, Brose N. Lipid binding defects and perturbed synaptogenic activity of a Collybistin R290H mutant that causes epilepsy and intellectual disability. J Biol Chem. 2015;290:8256-70 pubmed publisher
Brady M, Jacob T. Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation. Dev Neurobiol. 2015;75:1241-51 pubmed publisher
Flores C, Nikonenko I, Mendez P, Fritschy J, Tyagarajan S, Muller D. Activity-dependent inhibitory synapse remodeling through gephyrin phosphorylation. Proc Natl Acad Sci U S A. 2015;112:E65-72 pubmed publisher
La Fata G, Gärtner A, Domínguez Iturza N, Dresselaers T, Dawitz J, Poorthuis R, et al. FMRP regulates multipolar to bipolar transition affecting neuronal migration and cortical circuitry. Nat Neurosci. 2014;17:1693-700 pubmed publisher
Dejanovic B, Schwarz G. Neuronal nitric oxide synthase-dependent S-nitrosylation of gephyrin regulates gephyrin clustering at GABAergic synapses. J Neurosci. 2014;34:7763-8 pubmed publisher
Petrini E, Ravasenga T, Hausrat T, Iurilli G, Olcese U, Racine V, et al. Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP. Nat Commun. 2014;5:3921 pubmed publisher
Han K, Holder J, Schaaf C, Lu H, Chen H, Kang H, et al. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature. 2013;503:72-7 pubmed publisher
Hoy J, Haeger P, Constable J, Arias R, McCallum R, Kyweriga M, et al. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. J Neurosci. 2013;33:9364-84 pubmed publisher
Schubert T, Hoon M, Euler T, Lukasiewicz P, Wong R. Developmental regulation and activity-dependent maintenance of GABAergic presynaptic inhibition onto rod bipolar cell axonal terminals. Neuron. 2013;78:124-37 pubmed publisher
Tyagarajan S, Ghosh H, Yevenes G, Imanishi S, Zeilhofer H, Gerrits B, et al. Extracellular signal-regulated kinase and glycogen synthase kinase 3? regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism. J Biol Chem. 2013;288:9634-47 pubmed publisher
Nair R, Lauks J, Jung S, Cooke N, de Wit H, Brose N, et al. Neurobeachin regulates neurotransmitter receptor trafficking to synapses. J Cell Biol. 2013;200:61-80 pubmed publisher
Su J, Stenbjorn R, Gorse K, Su K, Hauser K, Ricard Blum S, et al. Target-derived matricryptins organize cerebellar synapse formation through ?3?1 integrins. Cell Rep. 2012;2:223-30 pubmed publisher
Murk K, Wittenmayer N, Michaelsen Preusse K, Dresbach T, Schoenenberger C, Korte M, et al. Neuronal profilin isoforms are addressed by different signalling pathways. PLoS ONE. 2012;7:e34167 pubmed publisher
Varley Z, Pizzarelli R, Antonelli R, Stancheva S, Kneussel M, Cherubini E, et al. Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures. J Biol Chem. 2011;286:20942-51 pubmed publisher
Hoon M, Soykan T, Falkenburger B, Hammer M, Patrizi A, Schmidt K, et al. Neuroligin-4 is localized to glycinergic postsynapses and regulates inhibition in the retina. Proc Natl Acad Sci U S A. 2011;108:3053-8 pubmed publisher
Förstera B, Belaidi A, Jüttner R, Bernert C, Tsokos M, Lehmann T, et al. Irregular RNA splicing curtails postsynaptic gephyrin in the cornu ammonis of patients with epilepsy. Brain. 2010;133:3778-94 pubmed publisher
Ogino K, Ramsden S, Keib N, Schwarz G, Harvey R, Hirata H. Duplicated gephyrin genes showing distinct tissue distribution and alternative splicing patterns mediate molybdenum cofactor biosynthesis, glycine receptor clustering, and escape behavior in zebrafish. J Biol Chem. 2011;286:806-17 pubmed publisher
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton M, Südhof T. Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro. Science. 2010;329:1663-7 pubmed publisher
Waldvogel H, Baer K, Eady E, Allen K, Gilbert R, Mohler H, et al. Differential localization of gamma-aminobutyric acid type A and glycine receptor subunits and gephyrin in the human pons, medulla oblongata and uppermost cervical segment of the spinal cord: an immunohistochemical study. J Comp Neurol. 2010;518:305-28 pubmed publisher
Baer K, Waldvogel H, Faull R, Rees M. Localization of glycine receptors in the human forebrain, brainstem, and cervical spinal cord: an immunohistochemical review. Front Mol Neurosci. 2009;2:25 pubmed publisher
Smolinsky B, Eichler S, Buchmeier S, Meier J, Schwarz G. Splice-specific functions of gephyrin in molybdenum cofactor biosynthesis. J Biol Chem. 2008;283:17370-9 pubmed publisher
product information
Cat. Nr. :
147 111
Antigen :
Gephyrin
Synonyms :
Gphn
Host Species :
mouse
Tpye :
monoclonal
Form :
purified IgG
Cell Line :
3B11
Subtype :
IgG1
Immunogen :
Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)
Reactivity :
human, rat, mouse, zebrafish
WB :
WB
IP :
IP
ICC :
ICC
IHC :
IHC
ELISA :
ELISA
KO :
yes
Quantity :
100 µg
more info or order :
company information
Synaptic Systems
Rudolf-Wissell-Str. 28
37079 Goettingen
lab@sysy.com
http://www.sysy.com
49-551-50556-0
headquarters: germany