product summary
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company name :
Synaptic Systems
product type :
antibody
product name :
Gephyrin
catalog :
147 021
quantity :
300 µl
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
mAb7a
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 78
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 8b
Amegandjin C, Choudhury M, Jadhav V, Carriço J, Quintal A, Berryer M, et al. Sensitive period for rescuing parvalbumin interneurons connectivity and social behavior deficits caused by TSC1 loss. Nat Commun. 2021;12:3653 pubmed publisher
  • immunohistochemistry - free floating section; rat; loading ...; fig 5a
  • immunocytochemistry; rat; loading ...; fig 2a
  • western blot; rat; 1:1000; loading ...; fig s3
Kriebel M, Ebel J, Battke F, Griesbach S, Volkmer H. Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity. Front Mol Neurosci. 2020;13:43 pubmed publisher
  • immunohistochemistry - free floating section; mouse; loading ...; fig s1f
Cserép C, Pósfai B, Lénárt N, Fekete R, László Z, Lele Z, et al. Microglia monitor and protect neuronal function through specialized somatic purinergic junctions. Science. 2020;367:528-537 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9h4
Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, et al. Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:200; loading ...; fig 12j
  • immunocytochemistry; rat; 1:200; loading ...; fig 8e
Miralles C, Taylor M, Bear J, Fekete C, George S, Li Y, et al. Expression of protocadherin-γC4 protein in the rat brain. J Comp Neurol. 2019;: pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 1b
Han W, Li J, Pelkey K, Pandey S, Chen X, Wang Y, et al. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science. 2019;366:246-250 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3a
Duan J, Pandey S, Li T, Castellano D, Gu X, Li J, et al. Genetic Deletion of GABAA Receptors Reveals Distinct Requirements of Neurotransmitter Receptors for GABAergic and Glutamatergic Synapse Development. Front Cell Neurosci. 2019;13:217 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2a
Boccalaro I, Cristiá Lara L, Schwerdel C, Fritschy J, Rubi L. Cell type-specific distribution of GABAA receptor subtypes in the mouse dorsal striatum. J Comp Neurol. 2019;527:2030-2046 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig ev3a
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, et al. Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin. EMBO J. 2019;38: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4g
Martenson J, Yamasaki T, Chaudhury N, Albrecht D, Tomita S. Assembly rules for GABAA receptor complexes in the brain. elife. 2017;6: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 4d
Sohn J, Okamoto S, Kataoka N, Kaneko T, Nakamura K, Hioki H. Differential Inputs to the Perisomatic and Distal-Dendritic Compartments of VIP-Positive Neurons in Layer 2/3 of the Mouse Barrel Cortex. Front Neuroanat. 2016;10:124 pubmed publisher
  • immunocytochemistry; human; loading ...
Nguyen T, Schreiner D, Xiao L, Traunmüller L, Bornmann C, Scheiffele P. An alternative splicing switch shapes neurexin repertoires in principal neurons versus interneurons in the mouse hippocampus. elife. 2016;5: pubmed publisher
  • immunohistochemistry; rat; 1:2000; loading ...; fig s3
Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun. 2016;7:13365 pubmed publisher
  • immunohistochemistry; rat; 1:200; loading ...; fig 2d
Fekete C, Goz R, Dinallo S, Miralles C, Chiou T, Bear J, et al. In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex. J Comp Neurol. 2017;525:1291-1311 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 3
Xia M, Zhu S, Shevelkin A, Ross C, Pletnikov M. DISC1, astrocytes and neuronal maturation: a possible mechanistic link with implications for mental disorders. J Neurochem. 2016;138:518-24 pubmed publisher
  • immunohistochemistry; rat; loading ...; fig 5e
  • western blot; rat; loading ...; fig 2b
Um J, Choii G, Park D, Kim D, Jeon S, Kang H, et al. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation. J Biol Chem. 2016;291:10119-30 pubmed publisher
  • immunocytochemistry; mouse; fig 1
Zhang Q, Gao X, Li C, Feliciano C, Wang D, Zhou D, et al. Impaired Dendritic Development and Memory in Sorbs2 Knock-Out Mice. J Neurosci. 2016;36:2247-60 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 6
Lyons M, Chen L, Deng J, Finn C, Pfenning A, Sabhlok A, et al. The transcription factor calcium-response factor limits NMDA receptor-dependent transcription in the developing brain. J Neurochem. 2016;137:164-76 pubmed publisher
  • immunocytochemistry; rat; 1:200
  • immunohistochemistry; rat; 1:200
Fekete C, Chiou T, Miralles C, Harris R, Fiondella C, LoTurco J, et al. In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration. J Comp Neurol. 2015;523:1359-78 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3,4
  • immunocytochemistry; rat; 1:500; loading ...; fig 6
Kang Y, Ge Y, Cassidy R, Lam V, Luo L, Moon K, et al. A combined transgenic proteomic analysis and regulated trafficking of neuroligin-2. J Biol Chem. 2014;289:29350-64 pubmed publisher
  • immunocytochemistry; human
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, et al. Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis. J Neurosci. 2014;34:8151-63 pubmed publisher
  • immunocytochemistry; mouse; 1:250; fig 1
Karayannis T, Au E, Patel J, Kruglikov I, Markx S, Delorme R, et al. Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission. Nature. 2014;511:236-40 pubmed
  • immunohistochemistry; rat; 1:500
Corteen N, Cole T, Sarna A, Sieghart W, Swinny J. Localization of GABA-A receptor alpha subunits on neurochemically distinct cell types in the rat locus coeruleus. Eur J Neurosci. 2011;34:250-62 pubmed publisher
Halff E, Szulc B, Lesept F, Kittler J. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Rep. 2019;29:2599-2607.e6 pubmed publisher
Lorenz Guertin J, Bambino M, Das S, Weintraub S, Jacob T. Diazepam Accelerates GABAAR Synaptic Exchange and Alters Intracellular Trafficking. Front Cell Neurosci. 2019;13:163 pubmed publisher
Salib M, Joshi A, Katona L, Howarth M, Micklem B, Somogyi P, et al. GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3. J Neurosci. 2019;39:4527-4549 pubmed publisher
Binda C, Nakamura Y, Henley J, Wilkinson K. Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number. Biochem J. 2019;: pubmed publisher
Handara G, Hetsch F, Jüttner R, Schick A, Haupt C, Rathjen F, et al. The role of agrin, Lrp4 and MuSK during dendritic arborization and synaptogenesis in cultured embryonic CNS neurons. Dev Biol. 2019;445:54-67 pubmed publisher
Wang H, Cheng K, Chen P, Tseng K, Kwan A, Chang L. Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models. BMC Neurosci. 2018;19:72 pubmed publisher
Bragina L, Conti F. Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses. Front Neuroanat. 2018;12:74 pubmed publisher
Zhang P, Lu H, Peixoto R, Pines M, Ge Y, Oku S, et al. Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships. Cell. 2018;174:1450-1464.e23 pubmed publisher
He H, Shen W, Zheng L, Guo X, Cline H. Excitatory synaptic dysfunction cell-autonomously decreases inhibitory inputs and disrupts structural and functional plasticity. Nat Commun. 2018;9:2893 pubmed publisher
Okamoto K, Hitora Imamura N, Hioki H, Ikegaya Y. GABAergic malfunction in the anterior cingulate cortex underlying maternal immune activation-induced social deficits. J Neuroimmunol. 2018;321:92-96 pubmed publisher
Bae J, Lee J, Ko S, Cho Y, Rah J, Cho H, et al. Extrasynaptic homomeric glycine receptors in neurons of the rat trigeminal mesencephalic nucleus. Brain Struct Funct. 2018;223:2259-2268 pubmed publisher
Koh S, Chen W, Dejneka N, Harris I, Lu B, Girman S, et al. Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity. J Neurosci. 2018;38:2923-2943 pubmed publisher
Virtanen M, Lacoh C, Fiumelli H, Kosel M, Tyagarajan S, De Roo M, et al. Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex. Brain Struct Funct. 2018;223:1999-2012 pubmed publisher
Li J, Han W, Pelkey K, Duan J, Mao X, Wang Y, et al. Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development. Neuron. 2017;96:808-826.e8 pubmed publisher
Brady M, Pilli J, Lorenz Guertin J, Das S, Moon C, Graff N, et al. Depolarizing, inhibitory GABA type A receptor activity regulates GABAergic synapse plasticity via ERK and BDNF signaling. Neuropharmacology. 2018;128:324-339 pubmed publisher
de Groot C, Floriou Servou A, Tsai Y, Früh S, Kohler M, Parkin G, et al. RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of ?2 GABAA receptors. PLoS Genet. 2017;13:e1007073 pubmed publisher
Cantaut Belarif Y, Antri M, Pizzarelli R, Colasse S, Vaccari I, Soares S, et al. Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord. J Cell Biol. 2017;216:2979-2989 pubmed publisher
Glebov O, Jackson R, Winterflood C, Owen D, Barker E, Doherty P, et al. Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function. Cell Rep. 2017;18:2715-2728 pubmed publisher
Ripamonti S, Ambrozkiewicz M, Guzzi F, Gravati M, Biella G, Bormuth I, et al. Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. elife. 2017;6: pubmed publisher
Pennacchietti F, Vascon S, Nieus T, Rosillo C, Das S, Tyagarajan S, et al. Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation. J Neurosci. 2017;37:1747-1756 pubmed publisher
Abraira V, Kuehn E, Chirila A, Springel M, Toliver A, Zimmerman A, et al. The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell. 2017;168:295-310.e19 pubmed publisher
Früh S, Romanos J, Panzanelli P, Bürgisser D, Tyagarajan S, Campbell K, et al. Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells. J Neurosci. 2016;36:10296-10313 pubmed
Dondzillo A, Thompson J, Klug A. Recurrent Inhibition to the Medial Nucleus of the Trapezoid Body in the Mongolian Gerbil (Meriones Unguiculatus). PLoS ONE. 2016;11:e0160241 pubmed publisher
Gutierrez Mecinas M, Kuehn E, Abraira V, Polgár E, Watanabe M, Todd A. Immunostaining for Homer reveals the majority of excitatory synapses in laminae I-III of the mouse spinal dorsal horn. Neuroscience. 2016;329:171-81 pubmed publisher
Terauchi A, Johnson Venkatesh E, Bullock B, Lehtinen M, Umemori H. Retrograde fibroblast growth factor 22 (FGF22) signaling regulates insulin-like growth factor 2 (IGF2) expression for activity-dependent synapse stabilization in the mammalian brain. elife. 2016;5: pubmed publisher
Nakamura Y, Morrow D, Modgil A, Huyghe D, Deeb T, Lumb M, et al. Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged ?-Aminobutyric Acid Receptor, Type A (GABAA), ?2 Subunit Knock-in Mouse. J Biol Chem. 2016;291:12394-407 pubmed publisher
O Sullivan G, Jedlicka P, Chen H, Kalbouneh H, Ippolito A, Deller T, et al. Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice. Mol Cell Neurosci. 2016;72:101-13 pubmed publisher
Lenz M, Galanis C, Müller Dahlhaus F, Opitz A, Wierenga C, Szabó G, et al. Repetitive magnetic stimulation induces plasticity of inhibitory synapses. Nat Commun. 2016;7:10020 pubmed publisher
Saito A, Taniguchi Y, Rannals M, Merfeld E, Ballinger M, Koga M, et al. Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1. Mol Psychiatry. 2016;21:1449-59 pubmed publisher
Bhagat S, Butler S, Taylor J, McEwen B, Strittmatter S. Erasure of fear memories is prevented by Nogo Receptor 1 in adulthood. Mol Psychiatry. 2016;21:1281-9 pubmed publisher
Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao Cheng J, et al. A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling. Nat Commun. 2015;6:7222 pubmed publisher
Hausrat T, Muhia M, Gerrow K, Thomas P, Hirdes W, Tsukita S, et al. Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory. Nat Commun. 2015;6:6872 pubmed publisher
Fidzinski P, Korotkova T, Heidenreich M, Maier N, Schuetze S, Kobler O, et al. KCNQ5 K(+) channels control hippocampal synaptic inhibition and fast network oscillations. Nat Commun. 2015;6:6254 pubmed publisher
Flores C, Nikonenko I, Mendez P, Fritschy J, Tyagarajan S, Muller D. Activity-dependent inhibitory synapse remodeling through gephyrin phosphorylation. Proc Natl Acad Sci U S A. 2015;112:E65-72 pubmed publisher
Antonelli R, Pizzarelli R, Pedroni A, Fritschy J, Del Sal G, Cherubini E, et al. Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction. Nat Commun. 2014;5:5066 pubmed publisher
Petrini E, Ravasenga T, Hausrat T, Iurilli G, Olcese U, Racine V, et al. Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP. Nat Commun. 2014;5:3921 pubmed publisher
Mendoza Schulz A, Jing Z, Sánchez Caro J, Wetzel F, Dresbach T, Strenzke N, et al. Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse. EMBO J. 2014;33:512-27 pubmed publisher
Notter T, Panzanelli P, PFISTER S, Mircsof D, Fritschy J. A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system. Eur J Neurosci. 2014;39:165-75 pubmed publisher
Chugh D, Nilsson P, Afjei S, Bakochi A, Ekdahl C. Brain inflammation induces post-synaptic changes during early synapse formation in adult-born hippocampal neurons. Exp Neurol. 2013;250:176-88 pubmed publisher
Ganser L, Yan Q, James V, Kozol R, Topf M, Harvey R, et al. Distinct phenotypes in zebrafish models of human startle disease. Neurobiol Dis. 2013;60:139-51 pubmed publisher
Linsalata A, Chen X, Winters C, Reese T. Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments. J Comp Neurol. 2014;522:921-36 pubmed publisher
Stephan A, Madison D, Mateos J, Fraser D, Lovelett E, Coutellier L, et al. A dramatic increase of C1q protein in the CNS during normal aging. J Neurosci. 2013;33:13460-74 pubmed publisher
Bleckert A, Parker E, Kang Y, Pancaroglu R, Soto F, Lewis R, et al. Spatial relationships between GABAergic and glutamatergic synapses on the dendrites of distinct types of mouse retinal ganglion cells across development. PLoS ONE. 2013;8:e69612 pubmed publisher
Martinez Cue C, Martinez P, Rueda N, Vidal R, Garcia S, Vidal V, et al. Reducing GABAA ?5 receptor-mediated inhibition rescues functional and neuromorphological deficits in a mouse model of down syndrome. J Neurosci. 2013;33:3953-66 pubmed publisher
Pettem K, Yokomaku D, Takahashi H, Ge Y, Craig A. Interaction between autism-linked MDGAs and neuroligins suppresses inhibitory synapse development. J Cell Biol. 2013;200:321-36 pubmed publisher
Hughes D, Boyle K, Kinnon C, Bilsland C, Quayle J, Callister R, et al. HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus. Neuroscience. 2013;237:7-18 pubmed publisher
Li Y, Xiao H, Chiou T, Jin H, Bonhomme B, Miralles C, et al. Molecular and functional interaction between protocadherin-?C5 and GABAA receptors. J Neurosci. 2012;32:11780-97 pubmed publisher
Abbott C, Percival K, Martin P, Grünert U. Amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina. Vis Neurosci. 2012;29:157-68 pubmed publisher
Jackson J, Chugh D, Nilsson P, Wood J, Carlström K, Lindvall O, et al. Altered synaptic properties during integration of adult-born hippocampal neurons following a seizure insult. PLoS ONE. 2012;7:e35557 pubmed publisher
Soto F, Bleckert A, Lewis R, Kang Y, Kerschensteiner D, Craig A, et al. Coordinated increase in inhibitory and excitatory synapses onto retinal ganglion cells during development. Neural Dev. 2011;6:31 pubmed publisher
Hua Y, Sinha R, Thiel C, Schmidt R, Hüve J, Martens H, et al. A readily retrievable pool of synaptic vesicles. Nat Neurosci. 2011;14:833-9 pubmed publisher
Aroeira R, Ribeiro J, Sebastião A, Valente C. Age-related changes of glycine receptor at the rat hippocampus: from the embryo to the adult. J Neurochem. 2011;118:339-53 pubmed publisher
Takahashi H, Arstikaitis P, Prasad T, Bartlett T, Wang Y, Murphy T, et al. Postsynaptic TrkC and presynaptic PTP? function as a bidirectional excitatory synaptic organizing complex. Neuron. 2011;69:287-303 pubmed publisher
Suh Y, Terashima A, Petralia R, Wenthold R, Isaac J, Roche K, et al. A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking. Nat Neurosci. 2010;13:338-43 pubmed publisher
Kumar S, Buckmaster P. Hyperexcitability, interneurons, and loss of GABAergic synapses in entorhinal cortex in a model of temporal lobe epilepsy. J Neurosci. 2006;26:4613-23 pubmed
product information
Cat. Nr. :
147 021
Antigen :
Gephyrin
Synonyms :
Gphn
Host Species :
mouse
Tpye :
monoclonal
Form :
hybridoma supernatant
Cell Line :
mAb7a
Subtype :
IgG1
Immunogen :
Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Reactivity :
human, rat, mouse, pig, goldfish, zebrafish, frog
ICC :
ICC
IHC :
IHC
EM :
EM
KO :
yes
Quantity :
300 µl
more info or order :
company information
Synaptic Systems
Rudolf-Wissell-Str. 28
37079 Goettingen
lab@sysy.com
http://www.sysy.com
49-551-50556-0
headquarters: germany