product summary
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company name :
Synaptic Systems
product type :
antibody
product name :
VAChT
catalog :
139 103
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
/
reactivity :
mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - free floating section; mouse; loading ...; fig 1a
  • western blot; mouse; loading ...; fig s1b
Al Onaizi M, Parfitt G, Kolisnyk B, Law C, Guzman M, Barros D, et al. Regulation of Cognitive Processing by Hippocampal Cholinergic Tone. Cereb Cortex. 2017;27:1615-1628 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 9
Villette V, Guigue P, Picardo M, Sousa V, Leprince E, Lachamp P, et al. Development of early-born ?-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood. J Comp Neurol. 2016;524:2440-61 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:100; fig s8
Saunders A, Oldenburg I, Berezovskii V, Johnson C, Kingery N, Elliott H, et al. A direct GABAergic output from the basal ganglia to frontal cortex. Nature. 2015;521:85-9 pubmed publisher
Swaminathan M, Hill Yardin E, Bornstein J, Foong J. Endogenous Glutamate Excites Myenteric Calbindin Neurons by Activating Group I Metabotropic Glutamate Receptors in the Mouse Colon. Front Neurosci. 2019;13:426 pubmed publisher
Janickova H, Kljakic O, Rosborough K, Raulic S, Matovic S, Gros R, et al. Selective decrease of cholinergic signaling from pedunculopontine and laterodorsal tegmental nuclei has little impact on cognition but markedly increases susceptibility to stress. FASEB J. 2019;33:7018-7036 pubmed publisher
Boccalaro I, Cristiá Lara L, Schwerdel C, Fritschy J, Rubi L. Cell type-specific distribution of GABAA receptor subtypes in the mouse dorsal striatum. J Comp Neurol. 2019;527:2030-2046 pubmed publisher
Zhang L, Wu C, Martel D, West M, Sutton M, Shore S. Remodeling of cholinergic input to the hippocampus after noise exposure and tinnitus induction in Guinea pigs. Hippocampus. 2018;: pubmed publisher
Seigneur E, Polepalli J, Sudhof T. Cbln2 and Cbln4 are expressed in distinct medial habenula-interpeduncular projections and contribute to different behavioral outputs. Proc Natl Acad Sci U S A. 2018;115:E10235-E10244 pubmed publisher
Lv Y, Zhong S, Tang H, Luo B, Chen S, Chen L, et al. VEGF-A and VEGF-B Coordinate the Arteriogenesis to Repair the Infarcted Heart with Vagus Nerve Stimulation. Cell Physiol Biochem. 2018;48:433-449 pubmed publisher
Gillet C, Goyer D, Kurth S, Griebel H, Kuenzel T. Cholinergic innervation of principal neurons in the cochlear nucleus of the Mongolian gerbil. J Comp Neurol. 2018;526:1647-1661 pubmed publisher
Rodriguez Diaz R, Molano R, Weitz J, Abdulreda M, Berman D, Leibiger B, et al. Paracrine Interactions within the Pancreatic Islet Determine the Glycemic Set Point. Cell Metab. 2018;27:549-558.e4 pubmed publisher
Urban Ciecko J, Jouhanneau J, Myal S, Poulet J, Barth A. Precisely Timed Nicotinic Activation Drives SST Inhibition in Neocortical Circuits. Neuron. 2018;97:611-625.e5 pubmed publisher
Ritter K, Wang Z, Vezina C, Bjorling D, Southard Smith E. Serotonin Receptor 5-HT3A Affects Development of Bladder Innervation and Urinary Bladder Function. Front Neurosci. 2017;11:690 pubmed publisher
Hoover D, Brown T, Miller M, Schweitzer J, Williams D. Loss of Sympathetic Nerves in Spleens from Patients with End Stage Sepsis. Front Immunol. 2017;8:1712 pubmed publisher
Barry C, Ji E, Sharma H, Yap P, Spencer N, Matusica D, et al. Peptidergic nerve fibers in the urethra: Morphological and neurochemical characteristics in female mice of reproductive age. Neurourol Urodyn. 2017;: pubmed publisher
Li Y, Wang R, Qiao N, Peng G, Zhang K, Tang K, et al. Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells. J Biol Chem. 2017;292:19590-19604 pubmed publisher
Case D, Burton S, Gedeon J, Williams S, Urban N, Seal R. Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb. Nat Commun. 2017;8:652 pubmed publisher
Arumugam S, Garcera A, Soler R, Tabares L. Smn-Deficiency Increases the Intrinsic Excitability of Motoneurons. Front Cell Neurosci. 2017;11:269 pubmed publisher
Iulita M, Bistué Millón M, Pentz R, Aguilar L, Do Carmo S, Allard S, et al. Differential deregulation of NGF and BDNF neurotrophins in a transgenic rat model of Alzheimer's disease. Neurobiol Dis. 2017;108:307-323 pubmed publisher
Hoon M, Krishnamoorthy V, Gollisch T, Falkenburger B, Varoqueaux F. Loss of Neuroligin3 specifically downregulates retinal GABAA?2 receptors without abolishing direction selectivity. PLoS ONE. 2017;12:e0181011 pubmed publisher
Trevaskis J, Sacramento C, Jouihan H, Ali S, Le Lay J, Oldham S, et al. Neurturin and a GLP-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats. Diabetes. 2017;66:2007-2018 pubmed publisher
Janickova H, Rosborough K, Al Onaizi M, Kljakic O, Guzman M, Gros R, et al. Deletion of the vesicular acetylcholine transporter from pedunculopontine/laterodorsal tegmental neurons modifies gait. J Neurochem. 2017;140:787-798 pubmed publisher
Bott J, Héraud C, Cosquer B, Herbeaux K, Aubert J, Sartori M, et al. APOE-Sensitive Cholinergic Sprouting Compensates for Hippocampal Dysfunctions Due to Reduced Entorhinal Input. J Neurosci. 2016;36:10472-10486 pubmed
Roy A, Dakroub M, Tezini G, Liu Y, Guatimosim S, Feng Q, et al. Cardiac acetylcholine inhibits ventricular remodeling and dysfunction under pathologic conditions. FASEB J. 2016;30:688-701 pubmed publisher
Pallotto M, Watkins P, Fubara B, Singer J, Briggman K. Extracellular space preservation aids the connectomic analysis of neural circuits. elife. 2015;4: pubmed publisher
Frahm S, Antolin Fontes B, Görlich A, Zander J, Ahnert Hilger G, Ibañez Tallon I. An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence. elife. 2015;4:e11396 pubmed publisher
Wahba G, Schock S, Claridge E, Bettolli M, Grynspan D, Humphreys P, et al. MeCP2 in the enteric nervous system. Neurogastroenterol Motil. 2015;27:1156-61 pubmed publisher
Stangl H, Springorum H, Muschter D, Grässel S, Straub R. Catecholaminergic-to-cholinergic transition of sympathetic nerve fibers is stimulated under healthy but not under inflammatory arthritic conditions. Brain Behav Immun. 2015;46:180-91 pubmed publisher
Crittenden J, Lacey C, Lee T, Bowden H, Graybiel A. Severe drug-induced repetitive behaviors and striatal overexpression of VAChT in ChAT-ChR2-EYFP BAC transgenic mice. Front Neural Circuits. 2014;8:57 pubmed publisher
Biesemann C, Gr Nborg M, Luquet E, Wichert S, Bernard V, Bungers S, et al. Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO J. 2014;33:157-70 pubmed publisher
Iwabuchi S, Koh J, Wang K, Ho K, Harata N. Minimal Change in the cytoplasmic calcium dynamics in striatal GABAergic neurons of a DYT1 dystonia knock-in mouse model. PLoS ONE. 2013;8:e80793 pubmed publisher
Hua T, Yang T, Yang W, Liu K, Tang S. 3-D neurohistology of transparent tongue in health and injury with optical clearing. Front Neuroanat. 2013;7:36 pubmed publisher
Kolisnyk B, Al Onaizi M, Hirata P, Guzman M, Nikolova S, Barbash S, et al. Forebrain deletion of the vesicular acetylcholine transporter results in deficits in executive function, metabolic, and RNA splicing abnormalities in the prefrontal cortex. J Neurosci. 2013;33:14908-20 pubmed publisher
Olini N, Kurth S, Huber R. The effects of caffeine on sleep and maturational markers in the rat. PLoS ONE. 2013;8:e72539 pubmed publisher
Huang T, Hu J, Wang B, Nie Y, Geng J, Cheng L. Tlx3 controls cholinergic transmitter and Peptide phenotypes in a subset of prenatal sympathetic neurons. J Neurosci. 2013;33:10667-75 pubmed publisher
Kolisnyk B, Guzman M, Raulic S, Fan J, Magalhaes A, Feng G, et al. ChAT-ChR2-EYFP mice have enhanced motor endurance but show deficits in attention and several additional cognitive domains. J Neurosci. 2013;33:10427-38 pubmed publisher
Antes R, Ezra Elia R, Weinberger D, Solomon A, Ofri R, Michaelson D. ApoE4 induces synaptic and ERG impairments in the retina of young targeted replacement apoE4 mice. PLoS ONE. 2013;8:e64949 pubmed publisher
Wiese C, Ireland S, Fleming N, Yu J, Valerius M, Georgas K, et al. A genome-wide screen to identify transcription factors expressed in pelvic Ganglia of the lower urinary tract. Front Neurosci. 2012;6:130 pubmed publisher
Ma M, Luo M. Optogenetic activation of basal forebrain cholinergic neurons modulates neuronal excitability and sensory responses in the main olfactory bulb. J Neurosci. 2012;32:10105-16 pubmed publisher
Torres Benito L, Neher M, Cano R, Ruiz R, Tabares L. SMN requirement for synaptic vesicle, active zone and microtubule postnatal organization in motor nerve terminals. PLoS ONE. 2011;6:e26164 pubmed publisher
Nikolajsen G, Jensen M, West M. Cholinergic axon length reduced by 300 meters in the brain of an Alzheimer mouse model. Neurobiol Aging. 2011;32:1927-31 pubmed publisher
Rodriguez Diaz R, Dando R, Jacques Silva M, Fachado A, Molina J, Abdulreda M, et al. Alpha cells secrete acetylcholine as a non-neuronal paracrine signal priming beta cell function in humans. Nat Med. 2011;17:888-92 pubmed publisher
Martins Silva C, De Jaeger X, Guzman M, Lima R, Santos M, Kushmerick C, et al. Novel strains of mice deficient for the vesicular acetylcholine transporter: insights on transcriptional regulation and control of locomotor behavior. PLoS ONE. 2011;6:e17611 pubmed publisher
Schock S, Jolin Dahel K, Schock P, Staines W, Garcia Munoz M, Arbuthnott G. Striatal interneurons in dissociated cell culture. Histochem Cell Biol. 2010;134:1-12 pubmed publisher
Cea del Rio C, Lawrence J, Tricoire L, Erdelyi F, Szabo G, McBain C. M3 muscarinic acetylcholine receptor expression confers differential cholinergic modulation to neurochemically distinct hippocampal basket cell subtypes. J Neurosci. 2010;30:6011-24 pubmed publisher
Madhusudan A, Sidler C, Knuesel I. Accumulation of reelin-positive plaques is accompanied by a decline in basal forebrain projection neurons during normal aging. Eur J Neurosci. 2009;30:1064-76 pubmed publisher
de Castro B, De Jaeger X, Martins Silva C, Lima R, Amaral E, Menezes C, et al. The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009;29:5238-50 pubmed publisher
Podrygajlo G, Tegenge M, Gierse A, Paquet Durand F, Tan S, Bicker G, et al. Cellular phenotypes of human model neurons (NT2) after differentiation in aggregate culture. Cell Tissue Res. 2009;336:439-52 pubmed publisher
Fortune T, Lurie D. Chronic low-level lead exposure affects the monoaminergic system in the mouse superior olivary complex. J Comp Neurol. 2009;513:542-58 pubmed publisher
Martens H, Weston M, Boulland J, Grønborg M, Grosche J, Kacza J, et al. Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses. J Neurosci. 2008;28:13125-31 pubmed publisher
Gras C, Amilhon B, Lepicard E, Poirel O, Vinatier J, Herbin M, et al. The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone. Nat Neurosci. 2008;11:292-300 pubmed publisher
Wang B, Yang L, Wang Z, Zheng H. Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Proc Natl Acad Sci U S A. 2007;104:14140-5 pubmed
Hartig W, Stieler J, Boerema A, Wolf J, Schmidt U, Weissfuss J, et al. Hibernation model of tau phosphorylation in hamsters: selective vulnerability of cholinergic basal forebrain neurons - implications for Alzheimer's disease. Eur J Neurosci. 2007;25:69-80 pubmed
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W. Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis. J Neurochem. 2005;95:1732-45 pubmed
Rassadi S, Krishnaswamy A, Pié B, McConnell R, Jacob M, Cooper E. A null mutation for the alpha3 nicotinic acetylcholine (ACh) receptor gene abolishes fast synaptic activity in sympathetic ganglia and reveals that ACh output from developing preganglionic terminals is regulated in an activity-dependent retrograde ma. J Neurosci. 2005;25:8555-66 pubmed
Nishimaru H, Restrepo C, Ryge J, Yanagawa Y, Kiehn O. Mammalian motor neurons corelease glutamate and acetylcholine at central synapses. Proc Natl Acad Sci U S A. 2005;102:5245-9 pubmed
product information
Company Name :
Synaptic Systems
Cat. Nr. :
139 103
Antigen :
VAChT
Synonyms :
SLC18A3
Host Species :
rabbit
Tpye :
polyclonal
Form :
affinity purified
Immunogen :
Recombinant protein corresponding to AA 475 to 530 from rat VAChT (UniProt Id: Q62666)
Reactivity :
human, rat, mouse, pig
WB :
y
IP :
y
ICC :
y
IHC :
y
IHC P :
y
KO :
1
KD :
1
Quantity :
50 µg
more info or order :
company information
Synaptic Systems
Rudolf-Wissell-Str. 28
37079 Goettingen
lab@sysy.com
http://www.sysy.com
49-551-50556-0
headquarters: germany