product summary
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company name :
Synaptic Systems
product type :
antibody
product name :
SNAP25
catalog :
111 011
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
71.1
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; rat; loading ...
Ivanova D, Dobson K, Gajbhiye A, Davenport E, Hacker D, Ultanir S, et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...
Arias Hervert E, Xu N, Njus M, Murphy G, Hou Y, Williams J, et al. Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission. Physiol Rep. 2020;8:e14428 pubmed publisher
  • western blot; mouse; loading ...; fig 1f
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, et al. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science. 2019;363: pubmed publisher
  • western blot; mouse; 1:1000; fig 3i
Lüningschrör P, Binotti B, Dombert B, Heimann P, Pérez Lara A, Slotta C, et al. Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nat Commun. 2017;8:678 pubmed publisher
  • western blot; mouse; loading ...; fig 4
  • immunocytochemistry; rat; loading ...; fig 8a
Vazquez Cintron E, Beske P, Tenezaca L, Tran B, Oyler J, Glotfelty E, et al. Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery. Sci Rep. 2017;7:42923 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 5a
Wolfes A, Ahmed S, Awasthi A, Stahlberg M, Rajput A, Magruder D, et al. A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes. J Gen Physiol. 2017;149:149-170 pubmed publisher
  • immunoprecipitation; rat; fig 4a
  • western blot; rat; 1:1000; fig 3a
Takeuchi S, Iwama S, Takagi H, Kiyota A, Nakashima K, Izumida H, et al. Tomosyn Negatively Regulates Arginine Vasopressin Secretion in Embryonic Stem Cell-Derived Neurons. PLoS ONE. 2016;11:e0164544 pubmed publisher
  • immunohistochemistry; rat; 1:2000; fig 4
Zhang S, Wang P, Ren L, Hu C, Bi J. Protective effect of melatonin on soluble A?1-42-induced memory impairment, astrogliosis, and synaptic dysfunction via the Musashi1/Notch1/Hes1 signaling pathway in the rat hippocampus. Alzheimers Res Ther. 2016;8:40 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4e
Toft Bertelsen T, Ziomkiewicz I, Houy S, Pinheiro P, Sørensen J. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell. 2016;27:3329-3341 pubmed
  • western blot; rat; fig 1
Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
Ruiter M, Houy S, Engholm Keller K, Graham M, Sørensen J. SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release. Mol Cell Neurosci. 2019;102:103452 pubmed publisher
Lazarevic V, Mantas I, Flais I, Svenningsson P. Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex. Int J Mol Sci. 2019;20: pubmed publisher
Cheng H, Chuang H. Rapid and Sensitive Nano-Immunosensors for Botulinum. ACS Sens. 2019;: pubmed publisher
Ruiter M, Kadkova A, Scheutzow A, Malsam J, Sollner T, Sørensen J. An Electrostatic Energy Barrier for SNARE-Dependent Spontaneous and Evoked Synaptic Transmission. Cell Rep. 2019;26:2340-2352.e5 pubmed publisher
Garland M, Babin B, Miyashita S, Loscher S, Shen Y, Dong M, et al. Covalent Modifiers of Botulinum Neurotoxin Counteract Toxin Persistence. ACS Chem Biol. 2019;14:76-87 pubmed publisher
Maidorn M, Olichon A, Rizzoli S, Opazo F. Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons. MAbs. 2019;11:305-321 pubmed publisher
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P. Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool. Neuropharmacology. 2018;143:38-48 pubmed publisher
Bello O, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski K, et al. Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis. Proc Natl Acad Sci U S A. 2018;115:E7624-E7631 pubmed publisher
Zheng Y, Shen W, Zhang J, Yang B, Liu Y, Qi H, et al. CRISPR interference-based specific and efficient gene inactivation in the brain. Nat Neurosci. 2018;21:447-454 pubmed publisher
Zhang S, Lebreton F, Mansfield M, Miyashita S, Zhang J, Schwartzman J, et al. Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium. Cell Host Microbe. 2018;23:169-176.e6 pubmed publisher
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, et al. Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits. Front Behav Neurosci. 2017;11:260 pubmed publisher
Salunkhe V, Ofori J, Gandasi N, Salö S, Hansson S, Andersson M, et al. MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles. Physiol Rep. 2017;5: pubmed publisher
Nakaya N, Sultana A, Tomarev S. Impaired AMPA receptor trafficking by a double knockout of zebrafish olfactomedin1a/b. J Neurochem. 2017;143:635-644 pubmed publisher
Acuna C, Liu X, Sudhof T. How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs. Neuron. 2016;91:792-807 pubmed publisher
Bacaj T, Wu D, Burré J, Malenka R, Liu X, Sudhof T. Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles. PLoS Biol. 2015;13:e1002267 pubmed publisher
Koo S, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, et al. Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission. Neuron. 2015;88:330-44 pubmed publisher
Ciani L, Marzo A, Boyle K, Stamatakou E, Lopes D, Anane D, et al. Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1. Nat Commun. 2015;6:8302 pubmed publisher
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, et al. Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice. J Neurosci. 2015;35:11514-31 pubmed publisher
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H. Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse. J Neurosci. 2015;35:8272-90 pubmed publisher
Burré J, Sharma M, Südhof T. α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation. Proc Natl Acad Sci U S A. 2014;111:E4274-83 pubmed publisher
Wilhelm B, Mandad S, Truckenbrodt S, Kr hnert K, Sch fer C, Rammner B, et al. Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science. 2014;344:1023-8 pubmed publisher
Liu X, Heidelberger R, Janz R. Phosphorylation of syntaxin 3B by CaMKII regulates the formation of t-SNARE complexes. Mol Cell Neurosci. 2014;60:53-62 pubmed publisher
Kirizs T, Kerti Szigeti K, Lorincz A, Nusser Z. Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells. Eur J Neurosci. 2014;39:1771-83 pubmed publisher
Vazquez Cintron E, Vakulenko M, Band P, Stanker L, Johnson E, Ichtchenko K. Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm. PLoS ONE. 2014;9:e85517 pubmed publisher
Mohrmann R, de Wit H, Connell E, Pinheiro P, Leese C, Bruns D, et al. Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering. J Neurosci. 2013;33:14417-30 pubmed publisher
Burré J, Sharma M, Sudhof T. Systematic mutagenesis of ?-synuclein reveals distinct sequence requirements for physiological and pathological activities. J Neurosci. 2012;32:15227-42 pubmed publisher
Kaeser Woo Y, Yang X, Sudhof T. C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis. J Neurosci. 2012;32:2877-85 pubmed publisher
Malarkey E, Parpura V. Temporal characteristics of vesicular fusion in astrocytes: examination of synaptobrevin 2-laden vesicles at single vesicle resolution. J Physiol. 2011;589:4271-300 pubmed publisher
Barth J, Zimmermann H, Volknandt W. SV31 is a Zn2+-binding synaptic vesicle protein. J Neurochem. 2011;118:558-70 pubmed publisher
Sharma M, Burr J, S dhof T. CSP? promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity. Nat Cell Biol. 2011;13:30-9 pubmed publisher
Yeh F, Dong M, Yao J, Tepp W, Lin G, Johnson E, et al. SV2 mediates entry of tetanus neurotoxin into central neurons. PLoS Pathog. 2010;6:e1001207 pubmed publisher
Hoopmann P, Punge A, Barysch S, Westphal V, B ckers J, Opazo F, et al. Endosomal sorting of readily releasable synaptic vesicles. Proc Natl Acad Sci U S A. 2010;107:19055-60 pubmed publisher
Lazzell D, Belizaire R, Thakur P, Sherry D, Janz R. SV2B regulates synaptotagmin 1 by direct interaction. J Biol Chem. 2004;279:52124-31 pubmed publisher
Wojcik S, Rhee J, Herzog E, Sigler A, Jahn R, Takamori S, et al. An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size. Proc Natl Acad Sci U S A. 2004;101:7158-63 pubmed
Nagy G, Reim K, Matti U, Brose N, Binz T, Rettig J, et al. Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron. 2004;41:417-29 pubmed
product information
Cat. Nr. :
111 011
Antigen :
SNAP25
Synonyms :
SNAP 25, SUP
Host Species :
mouse
Tpye :
monoclonal
Form :
purified IgG
Cell Line :
71.1
Subtype :
IgG1
Immunogen :
Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity :
human, rat, mouse, vertebrates, invertebrates, zebrafish
WB :
WB
IP :
IP
ICC :
ICC
IHC P :
IHC-P
EM :
EM
KO :
yes
Quantity :
50 µg
more info or order :
company information
Synaptic Systems
Rudolf-Wissell-Str. 28
37079 Goettingen
lab@sysy.com
http://www.sysy.com
49-551-50556-0
headquarters: germany