product summary
Loading...
company name :
StressMarq Biosciences
product type :
protein
product name :
Tau-412 (1N4R) Wild-Type Pre-formed Fibrils
catalog :
SPR-512
quantity :
100 µg
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPR-512 image 1
Representative TEM image of E. coli-expressed Tau 1N4R Pre-Formed Fibrils, 200nm scale. Negative stain transmission electron microscopy images of SPR-512 acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain.
image 2 :
StressMarq Biosciences SPR-512 image 2
In vitro seeding activity of human Tau-412 (1N4R) monomer in ThT assay. Tau 1N4R pre-formed fibrils seed fibril formation of human Tau-412 (1N4R) monomers over 72 hours. Reactions (100uL) shaken at 600 rpm in Greiner-Bio 96 Well Non-Binding Cell Culture Microplates, Black (Greiner-Bio Catalog #655900) at 37oC in the presence of 25 uM ThT and 10 uM heparin and read with an XPS Microplate Reader set at 450nmex/485nmem.
image 3 :
StressMarq Biosciences SPR-512 image 3
Sedimentation assay on E. coli expressed hTau-412 (1N4R) Pre-formed Fibrils. Samples were spun down at 15,000 x g, washed, and then spun down again. Fibril samples are prepared in denaturing conditions prior to running on the gel. SDS-PAGE analysis on a 12% Bis-Tris gel shows that the majority of the fibril prep shifts to insoluble post-fibrillization of monomers.
product information
Catalog No :
SPR-512
Product Name :
Tau-412 (1N4R) Wild-Type Pre-formed Fibrils
Description :
Human Recombinant Tau-412 (1N4R) Wild-Type Pre-formed Fibrils
Size :
100 µg
Research Area(s) :
Neuroscience Neurodegeneration Alzheimer's Disease Tangles & Tau
Alternative Name(s) :
Tau monomer, Tau protein monomer, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Truncated Tau Protein Monomer, Tau-E, Tau 412
Category :
Protein
Nature :
Recombinant
Swiss-Prot :
P10636-7
Applications :
WB SDS PAGE In vitro Assay
Species :
Human
Expression System :
E.coli
Protein Length :
412 aa
Amino Acid Sequence :
MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDT
DAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEAEEAG
IGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGAD
GKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSS
GEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPK
KVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGST
ENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPG
GGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKS
EKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRE
NAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDM
VDSPQLATLADEVSASLAKQGL
Purity :
>95%
Protein Size :
42.967 kDa
Conjugate :
No Tag
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.