Alpha Synuclein A53T Mutant Monomers (Type 1) | StressMarq Biosciences SPR-325 product information

product summary

Loading...

company name :

StressMarq Biosciences

product type :

protein

product name :

Alpha Synuclein A53T Mutant Monomers (Type 1)

catalog :

SPR-325

quantity :

100 µg

more info or order :

StressMarq Biosciences product webpage

citations: 7

Reference
Fujita K, Homma H, Jin M, Yoshioka Y, Jin X, Saito Y, et al. Mutant α-synuclein propagates via the lymphatic system of the brain in the monomeric state. Cell Rep. 2023;42:112962 pubmed publisher
Ghosh A, Verma D, Cabrera G, Ofori K, Hernandez Quijada K, Kim J, et al. A Novel NOX Inhibitor Treatment Attenuates Parkinson's Disease-Related Pathology in Mouse Models. Int J Mol Sci. 2022;23: pubmed publisher
Hlushchuk I, Barut J, Airavaara M, Luk K, Domanskyi A, Chmielarz P. Cell Culture Media, Unlike the Presence of Insulin, Affect α-Synuclein Aggregation in Dopaminergic Neurons. Biomolecules. 2022;12: pubmed publisher
Bhatia T, Clark R, Needham P, Miner K, Jamenis A, Eckhoff E, et al. Heat Shock Protein 70 as a Sex-Skewed Regulator of α-Synucleinopathy. Neurotherapeutics. 2021;: pubmed publisher
Verma D, Seo B, Ghosh A, Ma S, Hernandez Quijada K, Andersen J, et al. Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson's Disease Models. Cells. 2021;10: pubmed publisher
Hlushchuk I, Ruskoaho H, Domanskyi A, Airavaara M, Välimäki M. Domain-Independent Inhibition of CBP/p300 Attenuates α-Synuclein Aggregation. ACS Chem Neurosci. 2021;12:2273-2279 pubmed publisher
Verma D, Ghosh A, Ruggiero L, Cartier E, Janezic E, Williams D, et al. The SUMO Conjugase Ubc9 Protects Dopaminergic Cells from Cytotoxicity and Enhances the Stability of α-Synuclein in Parkinson's Disease Models. Eneuro. 2020;7: pubmed publisher

image

image 1 :

SDS-PAGE of ~14 kDa A53T Alpha Synuclein Monomer (SPR-325)

image 2 :

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.

image 3 :

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (SPR-325). A much greater increase in fluorescence is seen when 100 uM monomer (SPR-325) is combined with 10 nM of fibrils (SPR-326) as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.

product information

Catalog No :

SPR-325

Product Name :

Alpha Synuclein A53T Mutant Monomers (Type 1)

Description :

Human Recombinant A53T Mutant Alpha Synuclein Protein Monomers (Type 1)

Size :

100 µg

Research Area(s) :

Neuroscience Neurodegeneration Alzheimer's Disease Tangles & Tau Neuroscience Neurodegeneration Parkinson's Disease Synuclein Neuroscience Neurodegeneration Multiple System Atrophy

Alternative Name(s) :

A53T mutant alpha synuclein, A53T mutated SNCA, A53T Alpha synuclein monomer, Ala53thr mutant alpha synuclein, Alpha synuclein protein, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson disease familial 1 Protein

Category :

Protein

Nature :

Recombinant

Accession Number :

NP_000336.1

Swiss-Prot :

P37840

Applications :

WB SDS-PAGE In vivo assay In vitro assay

Species :

Human

Expression System :

E. coli

Protein Length :

Full Length

Amino Acid Sequence :

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Purity :

>95%

Protein Size :

~14.46 kDa

Conjugate :

No tag

Cellular Localization :

Cytoplasm Membrane Nucleus

more info or order :

StressMarq Biosciences product webpage