product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
GLUT2 Antibody
catalog :
SPC-697D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-697D image 1
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody (SPC-697) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.
product information
Catalog No :
SPC-697D
Product Name :
GLUT2 Antibody
Description :
Rabbit Anti-Human GLUT2 Polyclonal
Target :
GLUT2
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Cancer Metabolism Cancer Lipid and Lipoprotein Metabolism Cancer Cancer Metabolism Cancer Carbohydrate Metabolism Stem Cells Lineage Markers Endoderm
Alternative Name(s) :
Glucose Transporter 2 Antibody, Glucose Transporter GLUT2 Antibody, BLUT-2 Antibody, GTR_Human Antibody, SLC2a2 Antibody, Solute Carrier Family 2 (facilitated glucose transporter) member 2 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the C-terminal of human GLUT2
Immunogen Species :
Human
Accession Number :
NP_000331.1
Swiss-Prot :
P11168
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Rt
Species Reactivity Full Name :
Human Rat
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~57.5kDa. Observed molecular weight at 60-70kDa and 38-45kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-697D, RRID: AB_2705861
Certificate of Analysis :
A 1:1000 dilution of SPC-697 was sufficient for detection of GLUT2 on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Membrane
Scientific Background :
Glucose transporter 2 (GLUT2) also known as solute carrier family 2 (facilitated glucose transporter), member 2 (SLC2A2) is a transmembrane carrier protein that enables protein facilitated glucose movement across cell membranes. It is the principal transporter for transfer of glucose between liver and blood, and has a role in renal glucose reabsorption (1). Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (2). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (3).
References :
1. Kellett G.L., Brot-Laroche E. (2005) Diabetes. 54(10): 3056-3062. 2. Sansbury F.H., et al. (2012) Diabetologia. 55(9): 2381-2385. 3. Su Z., et al. (2011) J Perdiatr Endocrinol Metab. 24(9-10): 749-753.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-697-GLUT2-Antibody-ICC-IF-Human-Colon-cancer-cell-line-HT-29-60X-Composite-1.png SPC-697_GLUT2_Antibody_WB_Rat_Liver_1.png SPC-697_GLUT2_Antibody_WB_Human_HeLa_293T_1.png
Image Alternative Text :
Rabbit Anti-GLUT2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Colon cancer cell line (HT-29) (SPC-697) Rabbit Anti-GLUT2 Antibody used in Western blot (WB) on Liver (SPC-697) Rabbit Anti-GLUT2 Antibody used in Western blot (WB) on HeLa and HEK293T cell lysates (SPC-697)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.