product summary
Loading...
company name :
StressMarq Biosciences
product type :
antibody
product name :
PCNA Antibody
catalog :
SPC-691D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
image
image 1 :
StressMarq Biosciences SPC-691D image 1
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.
image 2 :
StressMarq Biosciences SPC-691D image 2
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.
product information
Catalog No :
SPC-691D
Product Name :
PCNA Antibody
Description :
Rabbit Anti-Human PCNA Polyclonal
Target :
PCNA
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Tumor Biomarkers Tumor Antigens Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Synthesis
Alternative Name(s) :
cb16 Antibody, MGC8367 Antibody, PCNA_HUMAN Antibody, Polymerase delta accessory protein Antibody, DNA polymerase delta auxiliary protein Antibody, Proliferating cell nuclear antigen Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, PCNAR Antibody, Pcna/cyclin Antibody, HGCN8729 Antibody, wu:fb36g03 Antibody, PCNA Antibody, ATLD2 Antibody, fb36g03 Antibody, OTTHUMP00000030190 Antibody, Cyclin Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide of Human PCNA
Immunogen Species :
Human
Accession Number :
NP_002583.1
Swiss-Prot :
P12004
Applications :
WB
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~37 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-691D, RRID: AB_2705789
Certificate of Analysis :
A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-691-PCNA-Antibody-WB-Human-293-Trap-cell-lysates-1.png
Image Alternative Text :
Rabbit Anti-PCNA Antibody used in Western blot (WB) on Embryonic kidney epithelial cell line (HEK293T) lysate (SPC-691)
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.