product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
beta Actin Antibody
catalog :
SPC-687D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-687D image 1
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~41.7kDa beta Actin protein using Rabbit Anti-beta Actin Polyclonal Antibody (SPC-687). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-beta Actin Polyclonal Antibody (SPC-687) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~41.7kDa.
product information
Catalog No :
SPC-687D
Product Name :
beta Actin Antibody
Description :
Rabbit Anti-Human beta Actin Polyclonal
Target :
beta Actin
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Cytoskeleton Microfilaments Tags and Cell Markers Organelle Markers
Alternative Name(s) :
A X actin like protein Antibody, ACTB Antibody, ACTB_HUMAN Antibody, Actin Antibody, Actin cytoplasmic 1 Antibody, actin beta Antibody, Actx Antibody, Beta-actin Antibody, PS1TP5 binding protein 1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the mid-protein range of human beta Actin
Immunogen Species :
Human
Accession Number :
NP_001092.1
Swiss-Prot :
P60709
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~41.7kDa. A double band may be observed.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-687D, RRID: AB_2705735
Certificate of Analysis :
A 1:1000 dilution of SPC-687 was sufficient for detection of beta Actin on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytoskeleton
Scientific Background :
Beta-actin is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-687-Beta-Actin-Antibody-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-40X-Composite-1.png SPC-687_beta-Actin_Antibody_WB_Human_HeLa_293T_1.png
Image Alternative Text :
Rabbit Anti-Beta Actin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-687) Rabbit Anti-beta Actin Antibody used in Western blot (WB) on HeLa and HEK293T cell lysates (SPC-687)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.