product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
BNIP3 Antibody
catalog :
SPC-685D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).
product information
Catalog No :
SPC-685D
Product Name :
BNIP3 Antibody
Description :
Rabbit Anti-Human BNIP3 Polyclonal
Target :
BNIP3
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cardiovascular System Heart Cancer Apoptosis Cell Signaling Metabolism Metabolism processes Hypoxia Cell Signaling Metabolism Mitochondrial Metabolism Mitochondrial Markers
Alternative Name(s) :
NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the C-terminal of human BNIP3
Immunogen Species :
Human
Accession Number :
NP_004043.3
Swiss-Prot :
Q12983
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-685D, RRID: AB_2705699
Certificate of Analysis :
A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Mitochondrion Mitochondrion outer membrane
Scientific Background :
BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).
References :
1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351.
2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921.
3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-685-BNIP3-Antibody-ICC-IF-Human-Fibroblast-cell-line-NIH-3T3-60X-Composite-2.png SPC-685_BNIP3_Antibody_WB_Human_HeLa_293T_1.png SPC-685_BNIP3_Antibody_WB_Mouse_Kidney_1.png
Image Alternative Text :
Rabbit Anti-BNIP3 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Fibroblast cell line (NIH 3T3) (SPC-685) Rabbit Anti-BNIP3 Antibody used in Western blot (WB) on HeLa and HEK293T cell lysates (SPC-685) Rabbit Anti-BNIP3 Antibody used in Western blot (WB) on Kidney (SPC-685)
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
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