p53 Antibody | StressMarq Biosciences SPC-682D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p53 Antibody

catalog :

SPC-682D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (SPC-682). Lane 1: MW Ladder. Lane 2: Human A431 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (SPC-682) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.

product information

Catalog No :

SPC-682D

Product Name :

p53 Antibody

Description :

Rabbit Anti-Human p53 Polyclona

Target :

p53

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cancer Cell Cycle p53 Pathway Cancer Tumor suppressors Apoptosis Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair

Alternative Name(s) :

TP53 Antibody, Tumor Protein 53 Antibody, BCC7 Antibody, LFS1 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide from the C-terminal of human tumor protein p53.

Immunogen Species :

Human

Accession Number :

NP_000537.3

Swiss-Prot :

P04637

Applications :

WB ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Species Reactivity Full Name :

Human

Antibody Dilution :

WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Predicted molecular weight at ~43.7kDa. Observed bands at ~53kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-682D, RRID: AB_2705645

Certificate of Analysis :

A 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus PML body Endoplasmic Reticulum Mitochondrion matrix

Scientific Background :

The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).

References :

1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-682-p53-Antibody-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-40X-Composite-1.png SPC-682_p53_Antibody_WB_Human_HeLa_293T_1.png SPC-682_p53_Antibody_WB_Human_A431_1.png

Image Alternative Text :

Rabbit Anti-p53 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-682) Rabbit Anti-p53 Antibody used in Western blot (WB) on HeLa and HEK293T cell lysates (SPC-682) Rabbit Anti-p53 Antibody used in Western blot (WB) on A431 (SPC-682)

more info or order :

StressMarq Biosciences product webpage