product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Rubicon Antibody
catalog :
SPC-668D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~108 kDa Rubicon protein using Rabbit Anti-Rubicon Polyclonal Antibody (SPC-668). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody (SPC-668) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~108 kDa.
product information
Catalog No :
SPC-668D
Product Name :
Rubicon Antibody
Description :
Rabbit Anti-Human Rubicon Polyclonal
Target :
Rubicon
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cardiovascular System Heart Cancer Autophagy Cell Signaling Metabolism Metabolism processes
Alternative Name(s) :
Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Antibody, KIAA0226 Antibody, Rubicon Antibody, Baron Antibody, RUBCN Antibody, Beclin-1 associated RUN domain containing protein Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal of Human Rubicon
Immunogen Species :
Human
Accession Number :
NP_001139114.1
Swiss-Prot :
Q92622
Applications :
WB ICC/IF IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~95 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-668D, RRID: AB_2705519
Certificate of Analysis :
A 1:1000 dilution of SPC-668 was sufficient for detection of Rubicon in 15 µg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Late Endosome Lysosome Early Endosome
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-668-Rubicon-Antibody-WB-Human-HeLa-cell-lysates-1.png
SPC-668_Rubicon_Antibody_IHC_Human_Tons
il_20X_1.png
SPC-668_Rubicon_Antibody_ICC-IF_Human_Colon-carcinoma-cell-line-RKO_60X_Composite_1.png
SPC-668_Rubicon_Antibody_IHC_Human_Tons
il_20X_1.png
SPC-668_Rubicon_Antibody_ICC-IF_Human_Colon-carcinoma-cell-line-RKO_60X_Composite_1.png
Image Alternative Text :
Rabbit Anti-Rubicon Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Colon carcinoma cell line (RKO) (SPC-668) Rabbit Anti-Rubicon Antibody used in Immunohistochemistry (IHC) on Tonsil (SPC-668) Rabbit Anti-Rubicon Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-668)
more info or order :
company information

StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
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