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company name :
StressMarq Biosciences
product type :
antibody
product name :
Bcl-2 Antibody
catalog :
SPC-655D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-655D image 1
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~26.3 kDa Bcl-2 protein using Rabbit Anti-Bcl-2 Polyclonal Antibody (SPC-655). Lane 1: Molecular Weight Ladder (MW). Lane 2: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody (SPC-655) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~26.3 kDa.
product information
Catalog No :
SPC-655D
Product Name :
Bcl-2 Antibody
Description :
Rabbit Anti-Human Bcl-2 Polyclonal
Target :
Bcl-2
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Apoptosis Intracellular Bcl2 Family Cancer Apoptosis Mitochondrial Cancer Tumor Biomarkers Oncoproteins Cancer Cancer Metabolism Response to Hypoxia Cell Signaling Metabolism Mitochondrial Metabolism Mitochondrial Markers Oxidative Phosphorylation
Alternative Name(s) :
Protein phosphatase 1 regulatory subunit 50 Antibody, AW986256 Antibody, BCL2_HUMAN Antibody, Bcl2 Antibody, Apoptosis regulator Bcl2 Antibody, BCL2 Antibody, B cell leukemia/lymphoma 2 Antibody, Leukemia/lymphoma, B-cell, 2 Antibody, B cell CLL/lymphoma 2 Antibody, Bcl-2 Antibody, Oncogene B-cell leukemia 2 Antibody, D830018M01Rik Antibody, PPP1R50 Antibody, D630044D05Rik Antibody, Apoptosis regulator Bcl-2 Antibody, C430015F12Rik Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the mid-protein of Human Bcl-2
Immunogen Species :
Human
Accession Number :
NP_000624.2
Swiss-Prot :
P10415
Applications :
WB IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Rt
Species Reactivity Full Name :
Human Rat
Antibody Dilution :
WB (1:1000); IHC: (1:50); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~26 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-655D, RRID: AB_2705411
Certificate of Analysis :
A 1:1000 dilution of SPC-655 was sufficient for detection of Bcl-2 in 15 µg of human 293Trap cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Mitochondrion Outer Membrane Single-Pass Membrane Protein Nucleus Membrane Single-Pass Membrane Protein Endoplasmic Reticulum Membrane Single-Pass Membrane Protein
Tissue Specificity :
Expressed in a variety of tissues.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-655_Bcl-2_Antibody_IHC_Human_Colon_10X_1.png SPC-655_Bcl-2_Antibody_IHC_Human_Colon_10X_2.png SPC-655-Bcl-2-Antibody-WB-Human-293Trap-1.png
Image Alternative Text :
Rabbit Anti-Bcl-2 Antibody used in Immunohistochemistry (IHC) on Colon (SPC-655) Rabbit Anti-Bcl-2 Antibody used in Immunohistochemistry (IHC) on Colon (SPC-655) Rabbit Anti-Bcl-2 Antibody used in Western blot (WB) on Embryonic kidney epithelial cell line (HEK293T) lysate (SPC-655)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.