product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
WIPI2 Antibody
catalog :
SPC-649D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
image
image 1 :
StressMarq Biosciences SPC-649D image 1
Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody (SPC-649). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody (SPC-649) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.
product information
Catalog No :
SPC-649D
Product Name :
WIPI2 Antibody
Description :
Rabbit Anti-Human WIPI2 Polyclonal
Target :
WIPI2
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Autophagy
Alternative Name(s) :
WIPI2 Antibody, WD repeat domain phosphoinositide-interacting protein 2 Antibody, WIPI-2 Antibody, WIPI49-like protein 2 Antibody, CGI-50 Antibody, ATG21 Antibody, WIPI 2 Antibody, ATG18B Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the C-terminal of Human WIPI2
Immunogen Species :
Human
Accession Number :
NP_001028690.1
Swiss-Prot :
Q9Y4P8
Applications :
WB ICC/IF IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~55 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-649D, RRID: AB_2705357
Certificate of Analysis :
A 1:1000 dilution of SPC-649 was sufficient for detection of WIPI2 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Preautophagosomal Structure Membrane Peripheral Membrane Protein Cytoplasmic Side
Tissue Specificity :
Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-649-WIPI2-Antibody-WB-Human-HeLa-and-293Trap-cell-lysates-1.png
SPC-649_WIPI2_Antibody_IHC_Human_Heart_
20X_1.png
SPC-649_WIPI2_Antibody_ICC-IF_Human_HeLa-Cells-Human-Cervical-Cancer_60X_Composite_1.png
Image Alternative Text :
Rabbit Anti-WIPI2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-649) Rabbit Anti-WIPI2 Antibody used in Immunohistochemistry (IHC) on Heart (SPC-649) Rabbit Anti-WIPI2 Antibody used in Western blot (WB) on HeLa and HEK293Trap cell lysates (SPC-649)
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.