product summary
Loading...
company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG9B Antibody
catalog :
SPC-646D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry
image
image 1 :
StressMarq Biosciences SPC-646D image 1
Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.
product information
Catalog No :
SPC-646D
Product Name :
ATG9B Antibody
Description :
Rabbit Anti-Human ATG9B Polyclonal
Target :
ATG9B
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cardiovascular System Heart Cancer Autophagy Cell Signaling Metabolism Mitochondrial Metabolism
Alternative Name(s) :
APG9-like 2 Antibody, APG9L2 Antibody, Nitric oxide synthase 3-overlapping antisense gene protein Antibody, Protein sONE Antibody, NOS3AS Antibody, ATG9B Antibody, Autophagy-related protein 9B Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Immunogen Species :
Human
Accession Number :
NP_001303985.1
Swiss-Prot :
Q674R7
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~100 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-646D, RRID: AB_2705339
Certificate of Analysis :
A 1:1000 dilution of SPC-646 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasmic Vesicle Autophagosome Membrane Multi-Pass Membrane Protein
Tissue Specificity :
Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
Scientific Background :
ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.
References :
1. http://www.uniprot.org/uniprot/Q674R7
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-646_ATG9B_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-646-ATG9B-Antibody-WB-Rat-Brain-cell-lysates-1.png
Image Alternative Text :
Rabbit Anti-ATG9B Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-646) Rabbit Anti-ATG9B Antibody used in Western blot (WB) on Brain cell lysates (SPC-646)
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.