product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ULK3 Antibody
catalog :
SPC-642D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of Human HeLa lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa.
product information
Catalog No :
SPC-642D
Product Name :
ULK3 Antibody
Description :
Rabbit Anti-Human ULK3 Polyclonal
Target :
ULK3
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Protein Phosphorylation Serine / Threonine Kinases
Alternative Name(s) :
Serine/threonine-protein kinase ULK3 Antibody, EC:2.7.11.1 Antibody, Ulk3 Antibody, DKFZP434C131 Antibody, Unc-51-like kinase 3 Antibody, FLJ90566 Antibody, ULK3_HUMAN Antibody, unc 51 like kinase 3 (C. elegans) Antibody, ULK3 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177)
Immunogen Species :
Human
Accession Number :
NP_001092906.3
Swiss-Prot :
Q6PHR2
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~53 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-642D, RRID: AB_2705303
Certificate of Analysis :
A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Tissue Specificity :
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
Scientific Background :
UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2.
References :
1. Malovergan A., et al. (2010) J Biol Chem. 285(39): 30079-30090. 2. Young A.R., et al. (2009) Genes Dev. 23(7):798-803.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-642_ULK3_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-642-ULK3-Antibody-WB-Human-A549-cell-lysates-1.png SPC-642_ULK3_Antibody_WB_Human_HeLa-lysates_1.png
Image Alternative Text :
Rabbit Anti-ULK3 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-642) Rabbit Anti-ULK3 Antibody used in Western blot (WB) on Lung carcinoma epithelial cell line (A549) lysate (SPC-642) Rabbit Anti-ULK3 Antibody used in Western blot (WB) on HeLa lysates (SPC-642)
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
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