product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ULK1 Antibody
catalog :
SPC-640D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-640D image 1
Western blot analysis of Rat kidney lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysates. Load: 15 µg. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.
product information
Catalog No :
SPC-640D
Product Name :
ULK1 Antibody
Description :
Rabbit Anti-Human ULK1 Polyclonal
Target :
ULK1
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Signaling Cardiovascular System Heart Cell Signaling Protein Phosphorylation Serine / Threonine Kinases Cell Signaling Metabolism Mitochondrial Metabolism
Alternative Name(s) :
Unc-51 like kinase 1 (C. elegans) Antibody, Autophagy related protein 1 homolog Antibody, Autophagy-related protein 1 homolog Antibody, FLJ38455 Antibody, Serine/threonine-protein kinase ULK1 Antibody, Unc51.1 Antibody, ATG1A Antibody, UNC51, C. elegans, homolog of Antibody, EC:2.7.11.1 Antibody, Serine/threonine protein kinase Unc51.1 Antibody, FLJ46475 Antibody, ATG 1 Antibody, hATG1 Antibody, ATG1 Antibody, Unc-51-like kinase 1 Antibody, Serine/threonine protein kinase ULK1 Antibody, ULK1 Antibody, Unc 51 like kinase 1 Antibody, KIAA0722 Antibody, UNC 51 Antibody, ULK 1 Antibody, ULK1_HUMAN Antibody, UNC51 Antibody, Unc 51 (C. elegans) like kinase 1 Antibody, ATG1 autophagy related 1 homolog Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
Immunogen Species :
Human
Accession Number :
NP_003556.1
Swiss-Prot :
O75385
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~100 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-640D, RRID: AB_2705267
Certificate of Analysis :
A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytosol Preautophagosomal Structure
Tissue Specificity :
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Scientific Background :
UNC-51 like kinase 1 (ULK1) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking.
References :
1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12. 2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900. 3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13. 4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5. 5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-640_ULK1_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-640_ULK1_Antibody_WB_Human_HeLa-and-293Trap-cell-lysates_1.png SPC-640_ULK1_Antibody_WB_Rat_kidney-lysates_1.png
Image Alternative Text :
Rabbit Anti-ULK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-640) Rabbit Anti-ULK1 Antibody used in Western blot (WB) on HeLa and HEK293Trap cell lysates (SPC-640) Rabbit Anti-ULK1 Antibody used in Western blot (WB) on kidney lysates (SPC-640)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.