SQSTM1 Antibody | StressMarq Biosciences SPC-636D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

SQSTM1 Antibody

catalog :

SPC-636D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~65 kDa in all lysates.

product information

Catalog No :

SPC-636D

Product Name :

SQSTM1 Antibody

Description :

Rabbit Anti-Human SQSTM1 Polyclonal

Target :

SQSTM1

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cancer Autophagy Cell Signaling Protein Trafficking Vesicle Transport Epigenetics and Nuclear Signaling Cardiovascular System Heart

Alternative Name(s) :

EBI3-associated protein of 60 kDa Antibody, ORCA Antibody, Osi Antibody, SQSTM 1 Antibody, ZIP 3 Antibody, A170 Antibody, SQSTM1 Antibody, Ubiquitin binding protein p62 Antibody, Protein kinase C-zeta-interacting protein Antibody, FTDALS3 Antibody, Ubiquitin-binding protein p62 Antibody, PKC-zeta-interacting protein Antibody, EBIAP Antibody, Sqstm1 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI3 associated protein p60 Antibody, EBI3 associated protein of 60 kDa Antibody, SQSTM_HUMAN Antibody, OSF-6 Antibody, Paget disease of bone 3 Antibody, p62B Antibody, p60 Antibody, PDB 3 Antibody, EBI 3 associated protein p60 Antibody, MGC127197 Antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI 3 associated protein of 60 kDa Antibody, ZIP3 Antibody, STAP Antibody, p62 Antibody, Sequestosome 1 Antibody, OSIL Antibody, STONE14 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 Antibody, PDB3 Antibody, Oxidative stress induced like Antibody, Sequestosome-1 Antibody, ZIP Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide from the N-terminal to mid of Human SQSTM1 (aa. 195-205)

Immunogen Species :

Human

Accession Number :

NP_001135770.1

Swiss-Prot :

Q13501

Applications :

WB ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~60 kDa. Band at 40 kDa is isoform 2.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-636D, RRID: AB_2705213

Certificate of Analysis :

A 1:1000 dilution of SPC-636 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Late Endosome Lysosome Cytoplasmic Vesicle Autophagosome Nucleus Endoplasmic Reticulum Cytoplasm P-Body

Tissue Specificity :

Ubiquitously expressed.

Scientific Background :

SQSTM1 or Sequestome 1 (p62) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signaling proteins and triggering degradation of proteins throught the proteasome or lysossome. This gene regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8.

References :

1. Kirkin V., et al. (2009) Mol Cell. 34: 259-269. 2. Seibenhener M.L., et al. (2007) FEBS Lett. 581: 175-179. 3. Komatsu M., et al. (2010) Nat Cell Biol. 12: 213-223. 4. Bjørkøy G., et al. (2006) Autophagy. 2: 138-139. 5. Pankiv S., et al. (2007) J Biol Chem. 282: 24131-24145.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-636_SQSTM1_Antibody_ICC-IF_Mouse_NIH-3T3-Mouse-Fibroblast-cell-line_60X_Composite_1.png SPC-636-SQSTM1-Antibody-WB-Human-HeLa-and-293Trap-cell-lysates-1.png

Image Alternative Text :

Rabbit Anti-SQSTM1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Fibroblast cell line (NIH 3T3) (SPC-636) Rabbit Anti-SQSTM1 Antibody used in Western blot (WB) on HeLa and HEK293Trap cell lysates (SPC-636)

more info or order :

StressMarq Biosciences product webpage