product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG4D Antibody
catalog :
SPC-634D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-634D image 1
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~52.9kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-634). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-634) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.9kDa.
product information
Catalog No :
SPC-634D
Product Name :
ATG4D Antibody
Description :
Rabbit Anti-Human ATG4D Polyclonal
Target :
ATG4D
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cardiovascular System Heart Cancer Autophagy
Alternative Name(s) :
APG4 autophagy 4 homolog D Antibody, APG4 D Antibody, ATG4 autophagy related 4 homolog D Antibody, AUTL4 Antibody, Cysteine protease ATG4D Antibody, MGC31226 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the C-terminal of human ATG4D
Immunogen Species :
Human
Accession Number :
NP_001268433.1
Swiss-Prot :
Q86TL0
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~52kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-634D, RRID: AB_2705177
Certificate of Analysis :
A 1:1000 dilution of SPC-634 was sufficient for detection of ATG4D on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
References :
1. "Entrez Gene: Autophagy related 4D, cysteine peptidase". 2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-634-ATG4D-Antibody-ICC-IF-Human-Myoblast-cell-line-C2C12-60X-Composite-1.png SPC-634_ATG4D_Antibody_WB_Human_HeLa_1.png
Image Alternative Text :
Rabbit Anti-ATG4D Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Myoblast cell line (C2C12) (SPC-634) Rabbit Anti-ATG4D Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-634)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.