product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG4C Antibody
catalog :
SPC-632D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-632D image 1
Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (SPC-632). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (SPC-632) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
product information
Catalog No :
SPC-632D
Product Name :
ATG4C Antibody
Description :
Rabbit Anti-Human ATG4C Polyclonal
Target :
ATG4C
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Cardiovascular System Autophagy
Alternative Name(s) :
APG4 autophagy 4 homolog C Antibody, APG4 C Antibody, Atg4c Antibody, AUTL1 Antibody, Autophagin 3 Antibody, Cysteine protease ATG4C Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal of human ATG4C
Immunogen Species :
Human
Accession Number :
NP_116241.2
Swiss-Prot :
Q96DT6
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~52.5kDa. Observed molecular weights at ~45-60kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-632D, RRID: AB_2705141
Certificate of Analysis :
A 1:1000 dilution of SPC-632 was sufficient for detection of ATG4C on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
ATG4C belongs to the peptidase C54 family. It is a cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. It also has an activity of delipidating enzyme for the PE-conjugated forms (1).
References :
1. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-632-ATG4C-Antibody-ICC-IF-Human-Myoblast-cell-line-C2C12-60X-Composite-1.png SPC-632-ATG4C-Antibody-WB-Human-293T-1.png SPC-632-ATG4C-Antibody-WB-Rat-Liver-1.png
Image Alternative Text :
Rabbit Anti-ATG4C Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Myoblast cell line (C2C12) (SPC-632) Rabbit Anti-ATG4C Antibody used in Western blot (WB) on Embryonic kidney epithelial cell line (HEK293T) lysate (SPC-632) Rabbit Anti-ATG4C Antibody used in Western blot (WB) on Liver (SPC-632)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.