product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG4B Antibody
catalog :
SPC-630D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-630D image 1
Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band(s): Band appears at ~40 kDa.
product information
Catalog No :
SPC-630D
Product Name :
ATG4B Antibody
Description :
Rabbit Anti-Human ATG4B Polyclonal
Target :
ATG4B
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Autophagy Cardiovascular System Heart Cell Signaling
Alternative Name(s) :
MGC1353 Antibody, Autophagy-related cysteine endopeptidase 1 Antibody, ATG4B Antibody, hAPG4B Antibody, AUT-like 1 cysteine endopeptidase Antibody, Cysteine protease ATG4B Antibody, Autophagy-related protein 4 homolog B Antibody, Autophagin-1 Antibody, EC:3.4.22.- Antibody, ATG4B_HUMAN Antibody, APG4B Antibody, APG4 autophagy 4 homolog B Antibody, AUTL1 Antibody, KIAA0943 Antibody, Autophagy related cysteine endopeptidase 1 Antibody, Autophagin 1 Antibody, ATG4 autophagy related 4 homolog B (S. cerevisiae) Antibody, AUT like 1 cysteine endopeptidase Antibody, Autophagy related protein 4 homolog B Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)
Immunogen Species :
Human
Accession Number :
NP_037457.3
Swiss-Prot :
Q9Y4P1
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms
Species Reactivity Full Name :
Human Mouse
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~44 kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-630D, RRID: AB_2705105
Certificate of Analysis :
A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Tissue Specificity :
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Scientific Background :
Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation.
References :
1. Kabeya Y., et al. (2004) J Cell Sci. 117: 2805-2812. 2. Tanida I., et al. (2004) J Biol Chem. 279: 36268-36276. 3. Hemelaar J., et al. (2003) J Biol Chem. 278: 51841-51850.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-630_ATG4B_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-630-ATG4B-Antibody-WB-Mouse-Brain-cell-lysates-1.png
Image Alternative Text :
Rabbit Anti-ATG4B Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-630) Rabbit Anti-ATG4B Antibody used in Western blot (WB) on Brain cell lysates (SPC-630)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.