ATG4A Antibody | StressMarq Biosciences SPC-628D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

ATG4A Antibody

catalog :

SPC-628D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Rat Pancreas cell lysates showing detection of 45.3 kDa ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-628) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kDa. Other Band(s): ~55-60 kDa.

product information

Catalog No :

SPC-628D

Product Name :

ATG4A Antibody

Description :

Rabbit Anti-Human ATG4A Polyclonal

Target :

ATG4A

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cancer Autophagy Cardiovascular System Heart Cell Signaling

Alternative Name(s) :

ATG4 autophagy related 4 homolog A (S. cerevisiae) Antibody, MGC107179 Antibody, AV169859 Antibody, Autophagy-related cysteine endopeptidase 2 Antibody, hAPG4A Antibody, EC:3.4.22.- Antibody, ATG4A Antibody, Autophagin 2 Antibody, Autophagy related protein 4 homolog A Antibody, Cysteine protease ATG4A Antibody, AUTL2 Antibody, Autl2 Antibody, Autophagy-related protein 4 homolog A Antibody, Atg4al Antibody, Autophagin-2 Antibody, Autophagy related 4A cysteine peptidase Antibody, AUT like 2 cysteine endopeptidase Antibody, Autophagy related cysteine endopeptidase 2 Antibody, Apg4a Antibody, AUT-like 2 cysteine endopeptidase Antibody, APG4A Antibody, AI627006 Antibody, ATG4A_HUMAN Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide from the mid-protein of Human ATG4A (aa. 102-113)

Immunogen Species :

Human

Accession Number :

NP_001308216.1

Swiss-Prot :

Q8WYN0

Applications :

WB ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~45 kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-628D, RRID: AB_2705069

Certificate of Analysis :

A 1:1000 dilution of SPC-628 was sufficient for detection of ATG4A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm

Tissue Specificity :

Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.

Scientific Background :

Autophagy related 4A, cysteine peptidase (ATG4A or APG4A) is a C-54 cysteine protease that also belongs to the family of autophagin proteins, and is primarily found in skeletal muscle and brain. ATG4A is involved in cellular autophagy and has been studied as a target for cancer therapy. Specifically it cleaves GATE-16. Mutations in the ATG4a gene is critical for redox regulation and inhibits autophagosome formation.

References :

1. Mariño G., et al. (2003) J Biol Chem. 278: 3671-8. 2. Scherz-Shouval R., et al. (2003) J Biol Chem. 278: 14053-8. 3. Li M., et al. (2011) J Biol Chem. 286: 7327-38. 4. Scherz-Shouval R., et al. (2007) EMBO J. 26: 1749-60.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-628_ATG4A_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-628-ATG4A-Antibody-WB-Rat-Pancreas-cell-lysates-1.png

Image Alternative Text :

Rabbit Anti-ATG4A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-628) Rabbit Anti-ATG4A Antibody used in Western blot (WB) on Pancreas cell lysates (SPC-628)

more info or order :

StressMarq Biosciences product webpage