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company name :
StressMarq Biosciences
product type :
antibody
product name :
GABARAPL1 Antibody
catalog :
SPC-623D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-623D image 1
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~14kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~14kDa.
product information
Catalog No :
SPC-623D
Product Name :
GABARAPL1 Antibody
Description :
Rabbit Anti-Human GABARAPL1 Polyclonal
Target :
GABARAPL1
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Neurotransmission Neurotransmitter Receptors GABA Receptors Cancer Autophagy
Alternative Name(s) :
APG8 like Antibdoy, ATG8 Antibody, GABA(A) Receptor Associated Protein Like 1 Antibody, Glandular epithelial cell protein 1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the C-terminal of human GABARAPL1
Immunogen Species :
Human
Accession Number :
NP_113600.1
Swiss-Prot :
Q9H0R8
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Rt
Species Reactivity Full Name :
Human Rat
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~14-16kDa. Other observed bands at ~32kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-623D, RRID: AB_2705015
Certificate of Analysis :
A 1:1000 dilution of SPC-623 was sufficient for detection of GABARAPL1 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytoskeleton Endoplasmic Reticulum Golgi Apparatus Cytoplasmic Vesicle Autophagosome
Scientific Background :
GABARAPL1, one of the proteins belonging to the GABARAP (GABA(A) receptor-associated protein) family, is highly expressed in the central nervous system and implicated in processes such as receptor and vesicle transport as well as autophagy. The proteins that make up the GABARAP family demonstrate conservation of their amino acid sequences and protein structures. In humans, GABARAPL1 shares 86% identity with GABARAP and 61% with GABARAPL2 (GATE-16) (1). GABARAPL1, or GABA(A) Receptor-Associated Protein Like 1, is a ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (2).
References :
1. Le Grand J.N., at al. (2011) Autophagy. 7(11): 1302-1307. 2. Tanida S., et al. (2006) FEBS J. 273(11): 2553-2562.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-623-GABARAPL1-Antibody-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SPC-623_GABARAPL1_Antibody_WB_Human_HeLa_1.png
Image Alternative Text :
Rabbit Anti-GABARAPL1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-623) Rabbit Anti-GABARAPL1 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-623)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.