LC3A Antibody | StressMarq Biosciences SPC-613D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

LC3A Antibody

catalog :

SPC-613D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.

product information

Catalog No :

SPC-613D

Product Name :

LC3A Antibody

Description :

Rabbit Anti-Human LC3A Polyclonal

Target :

LC3A

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cancer Autophagy Cell Signaling Neuroscience Cardiovascular System Heart

Alternative Name(s) :

MAP1 light chain 3 like protein 1 Antibody, Map1lc3a Antibody, MAP1BLC3 Antibody, Autophagy-related ubiquitin-like modifier LC3 A Antibody, MAP1LC3A Antibody, LC3A Antibody, Microtubule-associated proteins 1A and 1B, light chain 3 Antibody, MAP1ALC3 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B light chain 3 A Antibody, Microtubule-associated protein 1 light chain 3 alpha Antibody, LC3 Antibody, Microtubule-associated proteins 1A/1B light chain 3A Antibody, Autophagy-related protein LC3 A Antibody, MAP1A/MAP1B LC3 A Antibody, ATG8E Antibody, MLP3A_HUMAN Antibody, MAP1 light chain 3-like protein 1 Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)

Immunogen Species :

Human

Accession Number :

NP_115903.1

Swiss-Prot :

Q9H492

Applications :

WB ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~14 kDa. Band at 50 kDa is LC3A complex.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-613D, RRID: AB_2704943

Certificate of Analysis :

A 1:1000 dilution of SPC-613 was sufficient for detection of LC3A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Cytoskeleton Endomembrane System Lipid-Anchor Cytoplasmic Vesicle Autophagosome Membrane Lipid-Anchor Cytoplasmic Vesicle Autophagosome

Tissue Specificity :

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Scientific Background :

Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.

References :

1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-613_LC3A_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-613-LC3A-Antibody-WB-Rat-Liver-cell-lysates-1.png

Image Alternative Text :

Rabbit Anti-LC3A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-613) Rabbit Anti-LC3A Antibody used in Western blot (WB) on Liver cell lysates (SPC-613)

more info or order :

StressMarq Biosciences product webpage