product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG5 Antibody
catalog :
SPC-611D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-611D image 1
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex.
product information
Catalog No :
SPC-611D
Product Name :
ATG5 Antibody
Description :
Rabbit Anti-Human ATG5 Polyclonal
Target :
ATG5
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Apoptosis Cancer Autophagy Cardiovascular System Heart Cell Signaling Metabolism Metabolism processes
Alternative Name(s) :
APG5-like Antibody, ASP Antibody, hAPG5 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the mid-protein of human ATG5
Immunogen Species :
Human
Accession Number :
NP_001273035.1
Swiss-Prot :
Q9H1Y0
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-611D, RRID: AB_2704907
Certificate of Analysis :
A 1:1000 dilution of SPC-611 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
References :
1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-611-ATG5-Antibody-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-40X-Composite-1.png SPC-611_ATG5_Antibody_WB_Human_HeLa_1.png
Image Alternative Text :
Rabbit Anti-ATG5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-611) Rabbit Anti-ATG5 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-611)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.