product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATG12 Antibody
catalog :
SPC-607D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-607D image 1
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.
product information
Catalog No :
SPC-607D
Product Name :
ATG12 Antibody
Description :
Rabbit Anti-Human ATG12 Polyclonal
Target :
ATG12
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cancer Autophagy Cell Signaling Epigenetics and Nuclear Signaling Cardiovascular System Heart
Alternative Name(s) :
APG12-like Antibody, APG12 Antibody, ATG12_Human Antibody, HAPG12 Antibody, FBR93 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the N-terminal of human ATG12
Immunogen Species :
Human
Accession Number :
NP_001264712.1
Swiss-Prot :
O94817
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~15.1kDa. Observed molecular weights at ~48-55kDa based on ATG12-ATG5 heterodimer.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-607D, RRID: AB_2704835
Certificate of Analysis :
A 1:1000 dilution of SPC-607 was sufficient for detection of ATG12 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
ATG12 (autophagy-related protein 12), also known as APG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the ATG12 family of proteins. ATG12 is a homolog of the yeast protein APG12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of APG12 covalently bound at the carboxy terminal glycine to lysine 149 of APG5. Similarly in humans, ATG12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, ATG10 and ATP (1-3). The ATG12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two ATG12 isoforms exist.
References :
1. Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. (1999) J Biol Chem. 273(51): 33889-33892. 2. Mizushuma N., et al. (1998) Nature. 395: 395-398. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-5981.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-607-ATG12-Antibody-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-40X-Composite-1.png SPC-607_ATG12_Antibody_WB_Human_HeLa_1.png
Image Alternative Text :
Rabbit Anti-ATG12 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-607) Rabbit Anti-ATG12 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-607)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.