product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Beclin 1 Antibody
catalog :
SPC-600D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-600D image 1
Western blot analysis of Rat Liver showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
product information
Catalog No :
SPC-600D
Product Name :
Beclin 1 Antibody
Description :
Rabbit Anti-Human Beclin 1 Polyclonal
Target :
Beclin 1
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Epigenetics and Nuclear Signaling Transcription Cancer Tumor suppressors Cancer Autophagy Cancer Cancer Metabolism
Alternative Name(s) :
APG6 Antibody, BCL-2 interacting protein beclin Antibody, Beclin 1 autophagy related Antibody, BECN1 Antibody, BECN1_HUMAN Antibody, GT197 Antibody, VPS30 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide from the mid-protein of human Beclin 1
Immunogen Species :
Human
Accession Number :
NP_001300927.1
Swiss-Prot :
Q14457
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Rt
Species Reactivity Full Name :
Human Rat
Antibody Dilution :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Predicted molecular weight at ~51kDa. Observed a secondary band on some lysates at ~30kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-600D, RRID: AB_2704709
Certificate of Analysis :
A 1:1000 dilution of SPC-600 was sufficient for detection of Beclin1 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Golgi apparatus Trans-Golgi network membrane Endosome Endoplasmic reticulum membrane Mitochondrion Mitochondrion membrane Cytoplasmic vesicle Autophagosome
Scientific Background :
Beclin 1 is the mammalian ortholog of the yeast autophagy -related gene Atg6. It regulates autophagy and has an important role in development, tumorigenesis and neurodegeneration (1). Beclin 1 is localized within cytoplasmic structures including the mitochondria, yet overexpression reveals some nuclear staining (2). Researchers have found that schizonphrenia is associated with low levels of Beclin-1 in the hippocampus (3). It may also protect against infection by a neuorvirulent strain of Sindbis virus (4).
References :
1. Zhong Y., et al. (2009) Nat Cell Biol. 11(4): 468-476. 2. Liang X.H., et al. (2001) Cancer Res. 61: 3443-3449. 3. Merenlender-Wagner A., et al. (2013) Mol. Psychiatry 20:126-132. 4. Liang X.H., et al. (1998) J Virol. 72: 8586-8596.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-600-Beclin-1-Antibody-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SPC-600_Beclin-1_Antibody_WB_Human_HeLa_1.png SPC-600_Beclin-1_Antibody_WB_Rat_Liver_1.png
Image Alternative Text :
Rabbit Anti-Beclin 1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-600) Rabbit Anti-Beclin 1 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-600) Rabbit Anti-Beclin 1 Antibody used in Western blot (WB) on Liver (SPC-600)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.