Aquaporin 4 Antibody | StressMarq Biosciences SPC-505D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Aquaporin 4 Antibody

catalog :

SPC-505D

quantity :

100 µg

price :

300.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 1	Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
	Sun Y, Zhang Y, Zhu Y, Zhang A, Huang S, Yin X, et al. Inhibition of mitochondrial complex-1 restores the downregulation of aquaporins in obstructive nephropathy. Am J Physiol Renal Physiol. 2016;311:F777-F786 pubmed publisher

image

image 1 :

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:2000.

product information

Catalog No :

SPC-505D

Product Name :

Aquaporin 4 Antibody

Description :

Rabbit Anti-Rat Aquaporin 4 Polyclonal

Target :

Aquaporin 4

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmission Ion Channels Aquaporins

Alternative Name(s) :

AQP 4 Antibody, AQP-4 Antibody, AQP4 Antibody, AQP4_HUMAN Antibody, Aquaporin type 4 Antibody, Aquaporin-4 Antibody, Aquaporin4 Antibody, HMIWC 2 Antibody, HMIWC2 Antibody, Mercurial insensitive water channel Antibody, Mercurial-insensitive water channel Antibody, MGC22454 Antibody, MIWC Antibody, WCH 4 Antibody, WCH4 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4

Immunogen Species :

Rat

Accession Number :

NP_036957.1

Swiss-Prot :

P47863

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~35kDa. May detect larger glycosylated bands ~50kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-505D, RRID: AB_10963604

Certificate of Analysis :

0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane

Scientific Background :

Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).

References :

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-505_Aquaporin-4_Antibody_IHC_Rat_kidney-tissue_1.png SPC-505_Aquaporin-4_Antibody_WB_Rat_kidney-tissue-lysates_1.png

Image Alternative Text :

Rabbit Anti-Aquaporin 4 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-505) Rabbit Anti-Aquaporin 4 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-505)

more info or order :

StressMarq Biosciences product webpage