Aquaporin 2 Antibody | StressMarq Biosciences SPC-503D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Aquaporin 2 Antibody

catalog :

SPC-503D

quantity :

100 µg

price :

300.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 2

Reference
Sun Y, Zhang Y, Zhu Y, Zhang A, Huang S, Yin X, et al. Inhibition of mitochondrial complex-1 restores the downregulation of aquaporins in obstructive nephropathy. Am J Physiol Renal Physiol. 2016;311:F777-F786 pubmed publisher
Erikci A, Ucar G, Yabanoglu Ciftci S. Role of serotonin in the regulation of renal proximal tubular epithelial cells. Ren Fail. 2016;38:1141-50 pubmed publisher

image

image 1 :

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503). Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody (SPC-503) at 1:2000. Showing glycosylated and non-glycosylated bands.

product information

Catalog No :

SPC-503D

Product Name :

Aquaporin 2 Antibody

Description :

Rabbit Anti-Rat Aquaporin 2 Polyclonal

Target :

Aquaporin 2

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmission Ion Channels Aquaporins

Alternative Name(s) :

ADH water channel Antibody, AQP 2 Antibody, AQP CD Antibody, AQP-2 Antibody, AQP-CD Antibody, AQP2 Antibody, AQP2_HUMAN Antibody, AQPCD Antibody, Aquaporin 2 collecting duct Antibody, Aquaporin CD Antibody, Aquaporin-2 Antibody, Aquaporin-CD Antibody, Aquaporin2 Antibody, Aquaporine 2 Antibody, Collecting duct water channel protein Antibody, MGC34501 Antibody, Water channel aquaporin 2 Antibody, Water channel protein for renal collecting duct Antibody, WCH CD Antibody, WCH-CD Antibody, WCHCD Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2

Immunogen Species :

Rat

Accession Number :

NP_037041.2

Swiss-Prot :

P34080

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~28.8kDa. May detect larger glycosylated bands ~35-50kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-503D, RRID: AB_10965071

Certificate of Analysis :

0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cell Membrane Apical Cell Membrane Basolateral Cell Membrane Cytoplasmic Vesicle Cytoplasmic Vesicle Membrane Golgi Apparatus

Scientific Background :

Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).

References :

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445. 4. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-503_Aquaporin-2_Antibody_IHC_Rat_kidney-tissue_1.png SPC-503_Aquaporin-2_Antibody_WB_Rat_kidney-tissue-lysates_1.png

Image Alternative Text :

Rabbit Anti-Aquaporin 2 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-503) Rabbit Anti-Aquaporin 2 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-503)

more info or order :

StressMarq Biosciences product webpage