ENaC gamma Antibody | StressMarq Biosciences SPC-405D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

ENaC gamma Antibody

catalog :

SPC-405D

quantity :

100 µg

price :

310.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 5c	Ferdaus M, Barber K, López Cayuqueo K, Terker A, Argaiz E, Gassaway B, et al. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol. 2016;594:4945-66 pubmed publisher
	Khedr S, Palygin O, Pavlov T, Blass G, Levchenko V, Alsheikh A, et al. Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrol. 2019;20:145 pubmed publisher
	Liu M, Zhu Y, Sun Y, Wen Z, Huang S, Ding G, et al. MnTBAP therapy attenuates the downregulation of sodium transporters in obstructive kidney disease. Oncotarget. 2018;9:394-403 pubmed publisher
	Zhang C, Wang L, Su X, Zhang J, Lin D, Wang W. ENaC and ROMK activity are inhibited in the DCT2/CNT of TgWnk4PHAII mice. Am J Physiol Renal Physiol. 2017;312:F682-F688 pubmed publisher
	Xu N, Hirohama D, Ishizawa K, Chang W, Shimosawa T, Fujita T, et al. Hypokalemia and Pendrin Induction by Aldosterone. Hypertension. 2017;69:855-862 pubmed publisher
	You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, et al. Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC. J Cell Sci. 2017;130:1027-1036 pubmed publisher
	Klemens C, Edinger R, Kightlinger L, Liu X, Butterworth M. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017;292:375-385 pubmed publisher
	Xu C, Lu A, Wang H, Fang H, Zhou L, Sun P, et al. (Pro)Renin receptor regulates potassium homeostasis through a local mechanism. Am J Physiol Renal Physiol. 2017;313:F641-F656 pubmed publisher
	Pavlov T, Levchenko V, Ilatovskaya D, Moreno C, Staruschenko A. Renal sodium transport in renin-deficient Dahl salt-sensitive rats. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed publisher
	Reed Geaghan E, Wright M, See L, Adelman P, Lee K, Koerber H, et al. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes. J Neurosci. 2016;36:4362-76 pubmed publisher
	Ramkumar N, Stuart D, Mironova E, Bugay V, Wang S, Abraham N, et al. Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport. Am J Physiol Renal Physiol. 2016;311:F186-94 pubmed publisher
	Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
	Xu W, Huang Y, Li L, Sun Z, Shen Y, Xing J, et al. Hyperuricemia induces hypertension through activation of renal epithelial sodium channel (ENaC). Metabolism. 2016;65:73-83 pubmed publisher

image

image 1 :

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-405). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-405) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.

product information

Catalog No :

SPC-405D

Product Name :

ENaC gamma Antibody

Description :

Rabbit Anti-Rat ENaC gamma Polyclonal

Target :

ENaC

Conjugate :

Unconjugated

2021 List Price :

310.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmission Ion Channels Sodium Channels Epithelial Sodium Channels (ENaC)

Alternative Name(s) :

SCNN1G Antibody, Epithelial Sodium Channel gamma Antibody, Amiloride sensitive epithelial sodium channel gamma subunit Antibody, Amiloride sensitive sodium channel subunit gamma Antibody, Amiloride-sensitive sodium channel subunit gamma Antibody, BESC3 Antibody, ENaC gamma subunit Antibody, ENaCG Antibody, ENaCgamma Antibody, Epithelial Na(+) channel subunit gamma Antibody, Epithelial Na+ channel subunit gamma Antibody, Gamma ENaC Antibody, Gamma NaCH Antibody, Gamma-ENaC Antibody, Gamma-NaCH Antibody, Nonvoltage gated sodium channel 1 subunit gamma Antibody, Nonvoltage-gated sodium channel 1 subunit gamma Antibody, PHA 1 Antibody, PHA1 Antibody, SCNEG Antibody, SCNN 1G Antibody, SCNN1G Antibody, SCNNG_HUMAN Antibody, Sodium channel non voltage gated 1 gamma subunit Antibody, Sodium channel nonvoltage gated 1 gamma Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)

Immunogen Species :

Rat

Accession Number :

NP_058742

Swiss-Prot :

P37091

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Rt Ms Xe Hm Hu

Species Reactivity Full Name :

Rat Mouse African clawed frog (Xenopus laevis) Hamster Human

Antibody Dilution :

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~83kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-405D, RRID: AB_10640369

Certificate of Analysis :

1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Apical Cell Membrane

Scientific Background :

The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

References :

1. Kakizoe Y., et al. (2009) J Hypertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-405_ENaC_Antibody_IHC_Rat_kidney-tissue_1.png SPC-405_ENaC_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-405_ENaC_Antibody_WB_Mouse_kidney-cortex_1.png

Image Alternative Text :

Rabbit Anti-ENaC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-405) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-405) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney cortex (SPC-405)

more info or order :

StressMarq Biosciences product webpage