product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
ENaC beta Antibody
catalog :
SPC-404D
quantity :
100 µg
price :
310.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
citations: 8
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 5b
Ferdaus M, Barber K, López Cayuqueo K, Terker A, Argaiz E, Gassaway B, et al. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol. 2016;594:4945-66 pubmed publisher
  • western blot; mouse; fig 1
Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
Khedr S, Palygin O, Pavlov T, Blass G, Levchenko V, Alsheikh A, et al. Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrol. 2019;20:145 pubmed publisher
You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, et al. Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC. J Cell Sci. 2017;130:1027-1036 pubmed publisher
Klemens C, Edinger R, Kightlinger L, Liu X, Butterworth M. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017;292:375-385 pubmed publisher
Xu C, Lu A, Wang H, Fang H, Zhou L, Sun P, et al. (Pro)Renin receptor regulates potassium homeostasis through a local mechanism. Am J Physiol Renal Physiol. 2017;313:F641-F656 pubmed publisher
Pavlov T, Levchenko V, Ilatovskaya D, Moreno C, Staruschenko A. Renal sodium transport in renin-deficient Dahl salt-sensitive rats. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed publisher
Ramkumar N, Stuart D, Mironova E, Bugay V, Wang S, Abraham N, et al. Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport. Am J Physiol Renal Physiol. 2016;311:F186-94 pubmed publisher
product information
Catalog No :
SPC-404D
Product Name :
ENaC beta Antibody
Description :
Rabbit Anti-Rat ENaC beta Polyclonal
Target :
ENaC
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Neurotransmission Ion Channels Sodium Channels Epithelial Sodium Channels (ENaC)
Alternative Name(s) :
SCNN1B Antibody, Amiloride sensitive sodium channel subunit beta Antibody, Amiloride-sensitive sodium channel beta-subunit Antibody, Beta ENaC Antibody, Beta NaCH Antibody, ENaC beta Antibody, ENaCB Antibody, Epithelial Na(+) channel subunit beta Antibody, Epithelial Na+ channel beta subunit Antibody, Epithelial Na+ channel subunit beta Antibody, Epithelial sodium channel beta 2 subunit Antibody, Epithelial sodium channel beta 3 subunit Antibody, Nonvoltage gated sodium channel 1 beta subunit Antibody, Nonvoltage gated sodium channel 1 subunit beta Antibody, Nonvoltage-gated sodium channel 1 beta subunit Antibody, SCNEB Antibody, SCNN 1B Antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) Antibody, Sodium channel nonvoltage gated 1 beta Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
Immunogen Species :
Rat
Accession Number :
NP_036780
Swiss-Prot :
P37090
Applications :
WB IHC ICC/IF IP
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt Xe Hm
Species Reactivity Full Name :
Human Mouse Rat African clawed frog (Xenopus laevis) Hamster
Antibody Dilution :
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~87kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-404D, RRID: AB_10644173
Certificate of Analysis :
1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Apical Cell Membrane
Scientific Background :
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
References :
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-404_ENaC_Antibody_IHC_Rat_kidney-tissue_1.png SPC-404_ENaC_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-404_ENaC_Antibody_WB_Mouse_mpkCCD-cell-lysates_1.png SPC-404_ENaC_Antibody_WB_Mouse_kidney-cortex_1.png
Image Alternative Text :
Rabbit Anti-ENaC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-404) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-404) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse mpkCCD cell lysates (SPC-404) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney cortex (SPC-404)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.