ENaC alpha Antibody | StressMarq Biosciences SPC-403D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

ENaC alpha Antibody

catalog :

SPC-403D

quantity :

100 µg

price :

310.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 12

Reference
Khedr S, Palygin O, Pavlov T, Blass G, Levchenko V, Alsheikh A, et al. Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrol. 2019;20:145 pubmed publisher
Hashimoto H, Nomura N, Shoda W, Isobe K, Kikuchi H, Yamamoto K, et al. Metformin increases urinary sodium excretion by reducing phosphorylation of the sodium-chloride cotransporter. Metabolism. 2018;85:23-31 pubmed publisher
Liu M, Zhu Y, Sun Y, Wen Z, Huang S, Ding G, et al. MnTBAP therapy attenuates the downregulation of sodium transporters in obstructive kidney disease. Oncotarget. 2018;9:394-403 pubmed publisher
Zhang C, Wang L, Su X, Zhang J, Lin D, Wang W. ENaC and ROMK activity are inhibited in the DCT2/CNT of TgWnk4PHAII mice. Am J Physiol Renal Physiol. 2017;312:F682-F688 pubmed publisher
You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, et al. Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC. J Cell Sci. 2017;130:1027-1036 pubmed publisher
Klemens C, Edinger R, Kightlinger L, Liu X, Butterworth M. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017;292:375-385 pubmed publisher
Xu C, Lu A, Wang H, Fang H, Zhou L, Sun P, et al. (Pro)Renin receptor regulates potassium homeostasis through a local mechanism. Am J Physiol Renal Physiol. 2017;313:F641-F656 pubmed publisher
Zheng W, Li X, Liu H, Wang Z, Hu Q, Li Y, et al. AMP-Activated Protein Kinase Attenuates High Salt-Induced Activation of Epithelial Sodium Channels (ENaC) in Human Umbilical Vein Endothelial Cells. Oxid Med Cell Longev. 2016;2016:1531392 pubmed publisher
Pavlov T, Levchenko V, Ilatovskaya D, Moreno C, Staruschenko A. Renal sodium transport in renin-deficient Dahl salt-sensitive rats. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed publisher
Ramkumar N, Stuart D, Mironova E, Bugay V, Wang S, Abraham N, et al. Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport. Am J Physiol Renal Physiol. 2016;311:F186-94 pubmed publisher
Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
Ramkumar N, Stuart D, Calquin M, Wang S, Niimura F, Matsusaka T, et al. Possible role for nephron-derived angiotensinogen in angiotensin-II dependent hypertension. Physiol Rep. 2016;4: pubmed publisher

image

image 1 :

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C)ENaC Antibody (D) Composite

product information

Catalog No :

SPC-403D

Product Name :

ENaC alpha Antibody

Description :

Rabbit Anti-Rat ENaC alpha Polyclonal

Target :

ENaC

Conjugate :

Unconjugated

2021 List Price :

310.00 USD

Currency :

USD

Research Area(s) :

Neuroscience Neurotransmission Ion Channels Sodium Channels Epithelial Sodium Channels (ENaC)

Alternative Name(s) :

SCNN1A Antibody, Epithelial Sodium Channel-α Antibody, Epithelial Sodium Channel alpha Antibody, Alpha ENaC 2 Antibody, Alpha ENaC Antibody, Alpha NaCH Antibody, Alpha-ENaC Antibody, Amiloride sensitive epithelial sodium channel alpha subunit Antibody, Amiloride sensitive sodium channel subunit alpha Antibody, Amiloride-sensitive sodium channel subunit alpha Antibody, ENaCa Antibody, ENaCalpha Antibody, Epithelial Na(+) channel subunit alpha Antibody, Epithelial Na+ channel subunit alpha Antibody, FLJ21883 Antibody, Nonvoltage gated sodium channel 1 subunit alpha Antibody, Nonvoltage-gated sodium channel 1 subunit alpha Antibody, SCNEA Antibody, SCNN 1 Antibody, SCNN1 Antibody, SCNN1A Antibody, SCNNA_HUMAN Antibody, Sodium channel nonvoltage gated 1 alpha Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Produced against a synthetic peptide mapping to the N-terminal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).

Immunogen Species :

Rat

Accession Number :

NP_113736

Swiss-Prot :

Q6IRJ1

Applications :

WB IHC ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Rt Ms Xe

Species Reactivity Full Name :

Human Rat Mouse African clawed frog (Xenopus laevis)

Antibody Dilution :

WB (1:1000), ICC/IF (1:400); IHC (1:25); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~85kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-403D, RRID: AB_10640131

Certificate of Analysis :

1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Apical Cell Membrane

Scientific Background :

The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

References :

1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-403_ENaC_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-403_ENaC_Antibody_WB_Mouse_kidney-tissue-lysates_1.png SPC-403_ENaC_Antibody_IHC_Rat_kidney-tissue_63X_1.png

Image Alternative Text :

Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-403) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney tissue lysates (SPC-403) Rabbit Anti-ENaC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-403)

more info or order :

StressMarq Biosciences product webpage