NCC Antibody | StressMarq Biosciences SPC-402D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

NCC Antibody

catalog :

SPC-402D

quantity :

100 µg

price :

310.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 1	Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
	Poulsen S, Limbutara K, Fenton R, Pisitkun T, Christensen B. RNA sequencing of kidney distal tubule cells reveals multiple mediators of chronic aldosterone action. Physiol Genomics. 2018;50:343-354 pubmed publisher
	Liu M, Zhu Y, Sun Y, Wen Z, Huang S, Ding G, et al. MnTBAP therapy attenuates the downregulation of sodium transporters in obstructive kidney disease. Oncotarget. 2018;9:394-403 pubmed publisher
	Poulsen S, Kristensen T, Brooks H, Kohan D, Rieg T, Fenton R. Role of adenylyl cyclase 6 in the development of lithium-induced nephrogenic diabetes insipidus. JCI Insight. 2017;2:e91042 pubmed publisher
	Poulsen S, Christensen B. Long-term aldosterone administration increases renal Na+-Cl- cotransporter abundance in late distal convoluted tubule. Am J Physiol Renal Physiol. 2017;313:F756-F766 pubmed publisher
	Xu C, Lu A, Wang H, Fang H, Zhou L, Sun P, et al. (Pro)Renin receptor regulates potassium homeostasis through a local mechanism. Am J Physiol Renal Physiol. 2017;313:F641-F656 pubmed publisher
	Procino G, Carmosino M, Milano S, Dal Monte M, Schena G, Mastrodonato M, et al. Î²3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function. Kidney Int. 2016;90:555-67 pubmed publisher
	Poulsen S, Praetorius J, Damkier H, Miller L, Nelson R, Hummler E, et al. Reducing Î±ENaC expression in the kidney connecting tubule induces pseudohypoaldosteronism type 1 symptoms during K+ loading. Am J Physiol Renal Physiol. 2016;310:F300-10 pubmed publisher

product information

Catalog No :

SPC-402D

Product Name :

NCC Antibody

Description :

Rabbit Anti-Rat NCC Polyclonal

Target :

NCC

Conjugate :

Unconjugated

2021 List Price :

310.00 USD

Currency :

USD

Research Area(s) :

Cancer Neuroscience Pumps/Transporters Ion Pumps/Transporters

Alternative Name(s) :

SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Immunogen Species :

Rat

Accession Number :

NP_062218, NP_000330

Swiss-Prot :

P55018, P55017

Applications :

WB IHC ICC/IF IEM

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Dg

Species Reactivity Full Name :

Human Mouse Rat Dog

Antibody Dilution :

WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~160kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-402D, RRID: AB_10641430

Certificate of Analysis :

1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane

Scientific Background :

NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

References :

1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-402_NCC_Antibody_IHC_Rat_kidney-tissue_2.png SPC-402_NCC_Antibody_WB_Rat_tissue-lysates_1.png SPC-402_NCC-Antibody-IHC-Mouse-kidney-tissue_1.png SPC-402_NCC-Antibody-IHC-Mouse-kidney-tissue_2.png

Image Alternative Text :

Rabbit Anti-NCC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-402) Rabbit Anti-NCC Antibody used in Western blot (WB) on Rat tissue lysates (SPC-402) Rabbit Anti-NCC Antibody used in Immunohistochemistry (IHC) on kidney tissue (SPC-402) Rabbit Anti-NCC Antibody used in Immunohistochemistry (IHC) on kidney tissue (SPC-402)

more info or order :

StressMarq Biosciences product webpage