product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
NKCC2 Antibody
catalog :
SPC-401D
quantity :
100 µg
price :
310.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
citations: 5
Reference
Maruyama H, Taguchi A, Nishikawa Y, Guili C, Mikame M, Nameta M, et al. Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice. FASEB J. 2018;32:4544-4559 pubmed publisher
Liu M, Zhu Y, Sun Y, Wen Z, Huang S, Ding G, et al. MnTBAP therapy attenuates the downregulation of sodium transporters in obstructive kidney disease. Oncotarget. 2018;9:394-403 pubmed publisher
Taguchi A, Nishinakamura R. Higher-Order Kidney Organogenesis from Pluripotent Stem Cells. Cell Stem Cell. 2017;21:730-746.e6 pubmed publisher
Yu G, Cheng M, Wang W, Zhao R, Liu Z. Involvement of WNK1-mediated potassium channels in the sexual dimorphism of blood pressure. Biochem Biophys Res Commun. 2017;485:255-260 pubmed publisher
Lu X, Wang F, Xu C, Soodvilai S, Peng K, Su J, et al. Soluble (pro)renin receptor via ?-catenin enhances urine concentration capability as a target of liver X receptor. Proc Natl Acad Sci U S A. 2016;113:E1898-906 pubmed publisher
product information
Catalog No :
SPC-401D
Product Name :
NKCC2 Antibody
Description :
Rabbit Anti-Rat NKCC2 Polyclonal
Target :
NKCC2
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Cancer Neuroscience Pumps/Transporters Ion Pumps/Transporters
Alternative Name(s) :
SLC12A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
Immunogen Species :
Rat
Accession Number :
NP_001257546.1
Swiss-Prot :
Q5PQV1
Applications :
WB IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~160kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-401D, RRID: AB_10640877
Certificate of Analysis :
1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Membrane
Scientific Background :
NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
References :
1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-401_NKCC2_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-401_NKCC2-Antibody-IHC-Rat-Kidney-63X_Composite-1.png
Image Alternative Text :
Rabbit Anti-NKCC2 Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-401) Rabbit Anti-NKCC2 Antibody used in Immunohistochemistry (IHC) on rat kidney (SPC-401)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.