product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
NHE3 Antibody
catalog :
SPC-400D
quantity :
100 µg
price :
310.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
citations: 4
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; fig 1
Jones F, Bailey M, Murray L, Lu Y, McNeilly S, Schlötzer Schrehardt U, et al. ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice. Dis Model Mech. 2016;9:165-76 pubmed publisher
Harris A, Lee H, Osis G, Fang L, Webster K, Verlander J, et al. Differences in renal ammonia metabolism in male and female kidney. Am J Physiol Renal Physiol. 2018;315:F211-F222 pubmed publisher
Lee H, Osis G, Handlogten M, Verlander J, Weiner I. Proximal tubule glutamine synthetase expression is necessary for the normal response to dietary protein restriction. Am J Physiol Renal Physiol. 2017;313:F116-F125 pubmed publisher
Pavlov T, Levchenko V, Ilatovskaya D, Moreno C, Staruschenko A. Renal sodium transport in renin-deficient Dahl salt-sensitive rats. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed publisher
product information
Catalog No :
SPC-400D
Product Name :
NHE3 Antibody
Description :
Rabbit Anti-Rat NHE3 Polyclonal
Target :
NHE3
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Neuroscience Pumps/Transporters Ion Pumps/Transporters Cancer
Alternative Name(s) :
Sodium / Hydrogen Exchanger 3 antibody, SLC9A3 Antibody, Na+/H+ exchanger 3 Antibody, isoform 3 Antibody, Solute carrier family 9 member 3 Antibody, NHE3MGC126720 Antibody, MGC126718 Antibody, solute carrier family 9 (sodium/hydrogen exchanger) member 3 Antibody, NHE-3 Antibody, Na(+)/H(+) exchanger 3 Antibody, Sodium/hydrogen exchanger 3 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide mapping to AA 809 to 831 of rat sequence
Immunogen Species :
Rat
Accession Number :
NP_036786
Swiss-Prot :
P26433
Applications :
WB ICC/IF IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Rt Ms
Species Reactivity Full Name :
Rat Mouse
Antibody Dilution :
WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~84kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-400D, RRID: AB_10643557
Certificate of Analysis :
1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Membrane
Tissue Specificity :
Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level)
Scientific Background :
Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
References :
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-400_NHE3_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-400_NHE3-Antibody-IHC-Rat-Colon-63X_Composite-1.png
Image Alternative Text :
Rabbit Anti-NHE3 Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-400) Rabbit Anti-NHE3 Antibody used in Immunohistochemistry (IHC) on Rat Colon (SPC-400)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.