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company name :
StressMarq Biosciences
product type :
antibody
product name :
HSP70B (Plant) Antibody
catalog :
SPC-315D
quantity :
100 µl
price :
314.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
Chlamydomonas reinhardtii
application :
western blot, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SPC-315D
Product Name :
HSP70B (Plant) Antibody
Description :
Rabbit Anti-Algae HSP70B (Plant) Polyclonal
Target :
HSP70
Conjugate :
Unconjugated
2021 List Price :
314.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
Alternative Name(s) :
HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Size :
100 µl
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA
Immunogen Species :
Algae
Accession Number :
XP_001696432.1
Swiss-Prot :
A8HYV3
Applications :
WB IP
Host Species :
Rabbit
Species Reactivity Abbreviation :
Alg Pl
Species Reactivity Full Name :
Algae Plant Algae (Chlamydomonas reinhardtii) Algae (Desmodesmus subspicatus) Moss (Physcomitrella patens)
Antibody Dilution :
WB (1:10000); optimal dilutions for assays should be determined by the user.
Purification :
Rabbit Antiserum
Storage Buffer :
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
Specificity :
Detects ~70kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-315D, RRID: AB_1501401
Cellular Localization :
Cytoplasm
Scientific Background :
HSP70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas chloroplasts. It interacts with HSP90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that using Chlamydomonas reinhardtii as plant model organism the alga encodes a HEP homolog (termed HEP2) that is localized to the stroma (4). HEP2 is expressed constitutively as a low abundance protein with an apparent molecular mass of ~21 kDa. In cell extracts HEP2 interacts with HSP70B in an ATP-dependent fashion. Coexpression of HSP70B with HEP2 in E. coli yields high levels of CGE1- binding competent HSP70B, which also displayed ATPase activity (4). Inactive HSP70B was more prone to proteolysis than active HSP70B. Although inactive HSP70B interacts with HEP2, it is not activated. Active HSP70B remains active for 48 h in the absence of HEP2, suggesting that HEP2 was not involved in maintaining HSP70B in an active state. However, it was found that some HSP70B expressed as a fusion protein with an N-terminal extension was activated when HEP2 was present during cleavage of the fusion protein, suggesting that in vivo HEP2 might be required for renewed folding of HSP70B after transit peptide cleavage (4). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
References :
1. Schroda M., Vallon O., Wollman F.A. and Beck C.F. (1999) Plant Cell. 11: 1165-1178. 2. Schroda M., et al. (2001) Plant Cell. 13: 2823-2839. 3. Liu C., et al. (2005) Mol Biol Cell. 16: 1165-1177. 4. Willund F., et al. (2008) J. Biol. Chem., 283(24): 16363-16373.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.