product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
HSP101/ClpB CT (Plant) Antibody
catalog :
SPC-305B
quantity :
200 µl
price :
314.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-305B image 1
Western blot analysis of Arabidopsis Arabidopsis thalina tissue showing detection of HSP101 protein using Rabbit Anti-HSP101 Polyclonal Antibody (SPC-305). Load: 2 µg. Primary Antibody: Rabbit Anti-HSP101 Polyclonal Antibody (SPC-305) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP-conjugated Goat Anti-Rabbit at 1:10000. From left to right: Arabidopsis thaliana WT stressed at 38°C for 1.5 hour (1); Hsp101 null mutant.  Band showing at ~101kDa.
product information
Catalog No :
SPC-305B
Product Name :
HSP101/ClpB CT (Plant) Antibody
Description :
Rabbit Anti-Plant HSP101/ClpB CT (Plant) Polyclonal
Target :
HSP101
Conjugate :
Unconjugated
2021 List Price :
314.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins
Alternative Name(s) :
heat shock protein 1010 Antibody, HOT1 Antibody, HSP101 Antibody, HSPS Antibody
Size :
200 µl
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Recombinant protein Arabidopsis thaliana HSP101/ClpB C-terminal
Immunogen Species :
Plant
Accession Number :
NP_565083.1
Swiss-Prot :
P42730
Applications :
WB
Host Species :
Rabbit
Species Reactivity Abbreviation :
Pl Ar
Species Reactivity Full Name :
Plant Arabidopsis thaliana Monocot (Agave tequilana)
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Rabbit Antiserum
Storage Buffer :
Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
Specificity :
Detects the C-terminal domain of HSP101 ~101kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-305B, RRID: AB_1501428
Certificate of Analysis :
1 µl/ml of SPC-305 was sufficient for detection of HSP101 in 2 µg of Arabidopsis thaliana WT lysate stressed at 38°C for 1.5 hours by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
HSPs/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (1, 2). HSP100 is one such major class that acts as a chaperone. HSP101/ClpB is a member of this HSP100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of HSP100 protein family are also expressed during seed development. HSP101 protein is localized in a cytoplasm.
References :
1. Vierling E. (1991) Annu Rev. Plant Physiol. Plant Mol. Biol. 42: 579-620. 2. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-305_HSP101_Antibody_WB_Arabidopsis_
Arabidopsis-thalina-tissue_1.png
Image Alternative Text :
Rabbit Anti-HSP101 Antibody used in Western blot (WB) on Arabidopsis Arabidopsis thalina tissue (SPC-305)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.