antibody

HSC70 (Salmon) Antibody

SPC-303D

100 µl

314.00 USD

polyclonal

domestic rabbit

nonconjugated

HSC70 (Salmon) Antibody

Rabbit Anti-Salmon HSC70 (Salmon) Polyclonal

HSC70

Unconjugated

314.00 USD

USD

Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers Cancer Heat Shock

HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody, HSPA8 Antibody

100 µl

Antibodies

Polyclonal

Synthetic peptide conjugated to KLH. The peptide target is specific to the HSC70 of salmonid fish.

Salmon

NP_001133079.1

B5DFX7

WB

Rabbit

Fs Slm

Fish Salmon Three-spined stickleback (Gasterosteus aculeatus)

WB (1:1000); optimal dilutions for assays should be determined by the user.

Rabbit Antiserum

Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.

Detects ~73kDa. The antibody detects HSC70, but not HSP70.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-303D, RRID: AB_1501426

Cytoplasm Melanosome

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5).

1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-303_HSC70_Antibody_WB_Salmon_tissue-lysates_1.png

Rabbit Anti-HSC70 Antibody used in Western blot (WB) on Salmon tissue lysates (SPC-303)