product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
BVR Antibody
catalog :
SPC-214D
quantity :
100 µg
price :
301.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-214D image 1
Western blot analysis of Human, Rat Brain cell lysates showing detection of BVR protein using Rabbit Anti-BVR Polyclonal Antibody (SPC-214). Lane 1: Rat Brain. Lane 2: Human Brain lysates. Load: 10 µg. Primary Antibody: Rabbit Anti-BVR Polyclonal Antibody (SPC-214) at 1:1000.
product information
Catalog No :
SPC-214D
Product Name :
BVR Antibody
Description :
Rabbit Anti-Human BVR Polyclonal
Target :
BVR
Conjugate :
Unconjugated
2021 List Price :
301.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Protein Phosphorylation Serine / Threonine Kinases MAPK Pathway Oxidative Stress Cell Signaling Metabolism Redox Metabolism
Alternative Name(s) :
Biliverdin Reductase Antibody, BIEA_HUMAN Antibody, Biliverdin IX alpha reductase Antibody, Biliverdin reductase A Antibody, Biliverdin-IX alpha-reductase Antibody, BLVR A Antibody, BLVR Antibody, Blvra Antibody, BVR A Antibody, BVRA Antibody, Zinc metalloprotein Antibody, zinc-metalloprotein Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Human native full-length BVR
Immunogen Species :
Human
Accession Number :
NP_000703.2
Swiss-Prot :
P53004
Applications :
WB IHC ICC/IF IP
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:2000), ICC/IF (1:120), IP (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~40-42kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-214D, RRID: AB_2704361
Certificate of Analysis :
1 µg/ml of SPC-214 was sufficient for detection of BVR in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin by converting a double bond between the second and third pyrrole ring into a single bond (1). It is ubiqutiously expressed in all tissues- it occurs in cells and brain regiuons that already display HO-1 and HO-2, but also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH7.0 and NADPH at pH8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3).
References :
1. Singleton J.W., Laster L. (1965). J Biol Chem. 240: 4780-4789. 2. Kutty R.K., Maines M.D. (1981) J Biol Chem. 256: 3956-3962. 3. Mishra M., Ndisand J.F. (2014) Curr Pharm Des. 20(9): 1370-1391.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-214_BVR_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-214-BVR-Antibody-WB-Human-Rat-Brain-cell-lysates-1.png
Image Alternative Text :
Rabbit Anti-BVR Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-214) Rabbit Anti-BVR Antibody used in Western blot (WB) on Human, Rat Brain cell lysates (SPC-214)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.