HO-2 Antibody | StressMarq Biosciences SPC-212D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HO-2 Antibody

catalog :

SPC-212D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Rat Brain cell lysates showing detection of HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212). Lane 1: Rat Brain lysate. Lane 2: Purified HO-2. Load: 10 µg. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody (SPC-212) at 1:1000.

product information

Catalog No :

SPC-212D

Product Name :

HO-2 Antibody

Description :

Rabbit Anti-Rat HO-2 Polyclonal

Target :

HO-2

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cancer Cancer Metabolism Response to Hypoxia Oxidative Stress Cell Signaling Metabolism Metabolism processes Hypoxia

Alternative Name(s) :

Heme oxygenase 2 Antibody, Heme oxygenase (decycling) 2 Antibody, Heme oxygenase (decyclizing) 2 Antibody, HMOX 2 Antibody, HMOX2 Antibody, HMOX2_HUMAN Antibody, HO 2 Antibody, HO2 antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Rat native full-length HO-2 purified from testes

Immunogen Species :

Rat

Accession Number :

NP_077363.1

Swiss-Prot :

P23711

Applications :

WB IHC IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:500), IHC (1:1000), IP (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~36kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-212D, RRID: AB_2704325

Certificate of Analysis :

2 µg/ml of SPC-212 was sufficient for detection of HO-2 in 20 µg of Rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Endoplasmic Reticulum Microsome

Scientific Background :

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

References :

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-212-HO-2-Antibody-IHC-Rat-Spinal-cord-1.png SPC-212-HO-2-Antibody-WB-Rat-Brain-cell-lysates-1.png

Image Alternative Text :

Rabbit Anti-HO-2 Antibody used in Immunohistochemistry (IHC) on Rat Spinal cord (SPC-212) Rabbit Anti-HO-2 Antibody used in Western blot (WB) on Rat Brain cell lysates (SPC-212)

more info or order :

StressMarq Biosciences product webpage