Cytochrome P450 Reductase Antibody | StressMarq Biosciences SPC-210D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Cytochrome P450 Reductase Antibody

catalog :

SPC-210D

quantity :

100 µg

price :

301.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Mouse lung lysate. Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only.

product information

Catalog No :

SPC-210D

Product Name :

Cytochrome P450 Reductase Antibody

Description :

Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal

Target :

Cytochrome P450 Reductase

Conjugate :

Unconjugated

2021 List Price :

301.00 USD

Currency :

USD

Research Area(s) :

Cardiovascular System Cell Signaling Metabolism Lipid and Lipoprotein Metabolism Cancer Cancer Metabolism Cancer Lipid and Lipoprotein Metabolism

Alternative Name(s) :

Cytochrome C Reductase Antibody, CCR Antibody, CPR Antibody, P450R Antibody, CYPOR Antibody, DKFZp686G04235 Antibody, FLJ26468 Antibody, NADPH Cytochrome Antibody, P450 Reductase Antibody, NADPH dependent cytochrome P450 reductase Antibody, NADPH-cytochrome P450 reductase Antibody, P450 (cytochrome) oxidoreductase Antibody, P450 Cytochrome Oxidoreductase Antibody, P450R Antibody, POR Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Rat native full-length Cytochrome P450 Reductase purified from liver tissue

Immunogen Species :

Rat

Accession Number :

NP_113764.1

Swiss-Prot :

P00388

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~77kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-210D, RRID: AB_2704289

Certificate of Analysis :

1 µg/ml of SPC-210 was sufficient for detection of Cytochrome P450 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Membrane Microsome

Scientific Background :

Cytochrome P450 Reductase is an enyme that is required for the transfer of electrons from NADPH to cytochrome P450 in microsomes. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 Reductase is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase (POR) have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis.

References :

1. Pandey A.V. and Flück C.E. (2013). Pharmacology & therapeutics 138 (2): 229–54. 2. Jensen K. and Møller BL (2010). Phytochemistry 71 (2-3): 132–41. 3. Flück C.E. et al. (2004). Nature Genetics 36 (3): 228–230.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-210_Cytochrome-P450-Reductase_Antib
ody_WB_Human_Mouse_Rat_Human_Mouse-and-
Rat-Lysates_1.png
SPC-210_Cytochrome-P450-Reductase_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-210_Cytochrome-P450-Reductase_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png

Image Alternative Text :

Rabbit Anti-Cytochrome P450 Reductase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-210) Rabbit Anti-Cytochrome P450 Reductase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-210) Rabbit Anti-Cytochrome P450 Reductase Antibody used in Western blot (WB) on Human, Mouse, Rat Human, Mouse and Rat Lysates (SPC-210)

more info or order :

StressMarq Biosciences product webpage