antibody

HSF1 Antibody

SPC-208D

100 µg

278.00 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunocytochemistry

HSF1 Antibody

Rabbit Anti-Human HSF1 Polyclonal

HSF1

Unconjugated

278.00 USD

USD

Cancer Heat Shock Cell Signaling Epigenetics and Nuclear Signaling Cardiovascular System Heart

Heat shock factor 1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody, hsf1 Antibody, HSF1_HUMAN Antibody, HSTF 1 Antibody, HSTF1 Antibody

100 µg

Antibodies

Polyclonal

Purified human HSF1 protein NM_005526.2

Human

NP_005517.1

Q00613

WB ICC/IF

Rabbit

Hu Ms Rt

Human Mouse Rat

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Protein A Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~85kDa (unstressed cell lysates), and ~95kDa (heat shocked cell lysates).

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-208D, RRID: AB_2704253

1 µg/ml of SPC-208 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Cytoplasm Nucleus

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-208_HSF1-Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-208_HSF1-Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-208) Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-208)