SOD1 (EDI) Antibody | StressMarq Biosciences SPC-206D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

SOD1 (EDI) Antibody

catalog :

SPC-206D

quantity :

100 µg

price :

278.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 2

Reference
Trist B, Davies K, Cottam V, Genoud S, Ortega R, Roudeau S, et al. Amyotrophic lateral sclerosis-like superoxide dismutase 1 proteinopathy is associated with neuronal loss in Parkinson's disease brain. Acta Neuropathol. 2017;134:113-127 pubmed publisher
Tokuda E, Anzai I, Nomura T, Toichi K, Watanabe M, Ohara S, et al. Immunochemical characterization on pathological oligomers of mutant Cu/Zn-superoxide dismutase in amyotrophic lateral sclerosis. Mol Neurodegener. 2017;12:2 pubmed publisher

image

image 1 :

Western blot analysis of Mouse Lung showing detection of ~18 kDa SOD1 (EDI) protein using Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206) at 1:1000. Predicted/Observed Size: ~18 kDa.

image 2 :

product information

Catalog No :

SPC-206D

Product Name :

SOD1 (EDI) Antibody

Description :

Rabbit Anti-Human SOD1 (EDI) Polyclonal

Target :

SOD1

Conjugate :

Unconjugated

2021 List Price :

278.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Neuroscience Neurodegeneration ALS Disease

Alternative Name(s) :

SEDI Antibody, SOD1 EDI Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 exposed dimer interface Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

C-terminal region of SOD1, exposed dimer interface (EDI)

Immunogen Species :

Human

Accession Number :

CAG46542

Swiss-Prot :

P00441

Applications :

WB IHC ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Pg

Species Reactivity Full Name :

Human Mouse Rat Pig

Antibody Dilution :

WB (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Recognizes a conformation specific epitope where the dimer interface is exposed.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-206D, RRID: AB_2704217

Certificate of Analysis :

1µg/ml of SPC-206 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm

Scientific Background :

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1.

References :

1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O'Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-206-SOD1-EDI-Antibody-WB-Mouse-Lung-1.png

Image Alternative Text :

Rabbit Anti-SOD1 (EDI) Antibody used in Western blot (WB) on Mouse Lung (SPC-206)

more info or order :

StressMarq Biosciences product webpage