TLR4 Antibody | StressMarq Biosciences SPC-200D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

TLR4 Antibody

catalog :

SPC-200D

quantity :

100 µg

price :

268.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

StressMarq Biosciences product webpage

citations: 2

Reference
Yanagawa M, Uchida K, Ando Y, Tomiyama T, Yamaguchi T, Ikeura T, et al. Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis. J Gastroenterol. 2018;53:449-460 pubmed publisher
Kuwabara Y, Katayama A, Kurihara S, Ito M, Yonezawa M, Ouchi N, et al. Diversity of progesterone action on lipopolysaccharide-induced expression changes in cultured human cervical fibroblasts according to inflammation and treatment timing. Am J Reprod Immunol. 2017;78: pubmed publisher

product information

Catalog No :

SPC-200D

Product Name :

TLR4 Antibody

Description :

Rabbit Anti-Human TLR4 Polyclonal

Target :

TLR4

Conjugate :

Unconjugated

2021 List Price :

268.00 USD

Currency :

USD

Research Area(s) :

Immunology Innate Immunity Macrophage Immunology Immunoglobulins Immunoglobulin Receptors Cardiovascular System Atherosclerosis Vascular Inflammation Inflammatory Mediators Immunology Adaptive Immunity Regulatory T Cells Immunology Innate Immunity TLR Signaling

Alternative Name(s) :

ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Immunogen Species :

Human

Accession Number :

NP_612564.1

Swiss-Prot :

O00206

Applications :

WB IHC ICC/IF FCM FACS

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms

Species Reactivity Full Name :

Human Mouse

Antibody Dilution :

WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-200D, RRID: AB_2204998

Certificate of Analysis :

2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane

Scientific Background :

The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).

References :

1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-200_TLR4_Antibody_IHC_Mouse_colon-colitis_1.png SPC-200_TLR4_Antibody_WB_Human_Mouse_Cell-line-lysates_1.png SPC-200_TLR4_Antibody_FCM_Human_Monocytic-Leukemia-cells-THP-1_1.png SPC-200_TLR4_Antibody_IHC_Mouse_Spleen-tissue_1.png SPC-200_TLR4_Antibody_IHC_Mouse_spleen-tissue_2.png

Image Alternative Text :

Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse colon colitis (SPC-200) Rabbit Anti-TLR4 Antibody used in Western blot (WB) on Human, Mouse Cell line lysates (SPC-200) Rabbit Anti-TLR4 Antibody used in Flow Cytometry (FCM) on Human Monocytic Leukemia cells (THP-1) (SPC-200) Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse Spleen tissue (SPC-200) Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse spleen tissue (SPC-200)

more info or order :

StressMarq Biosciences product webpage