product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Piccolo Antibody
catalog :
SPC-197D
quantity :
100 µg
price :
300.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-197D image 1
Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000.
product information
Catalog No :
SPC-197D
Product Name :
Piccolo Antibody
Description :
Rabbit Anti-Human Piccolo Polyclonal
Target :
Piccolo
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Presynaptic Markers
Alternative Name(s) :
ACZ Antibody, Aczonin Antibody, Brain-derived HLMN protein Antibody, DKFZp779G1236 Antibody, KIAA0559 Antibody, Multidomain presynaptic cytomatrix protein Antibody, PCLO Antibody, PCLO_HUMAN Antibody, Piccolo (presynaptic cytomatrix protein) Antibody, Piccolo mouse homolog of Pico Antibody, Presynaptic cytomatrix protein Antibody, Protein piccolo Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Full length protein, BC001304.1
Immunogen Species :
Human
Accession Number :
NP_055325.2
Swiss-Prot :
Q9Y6V0
Applications :
WB IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~550kDa. No cross-reactivity to Bassoon.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-197D, RRID: AB_10804537
Certificate of Analysis :
1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cell Junction Synapse
Scientific Background :
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
References :
1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-197_Piccolo_Antibody_IHC_Mouse_muscle_1.png SPC-197_Piccolo_Antibody_WB_Mouse_Rat_brain-cell-lysates_1.png
Image Alternative Text :
Rabbit Anti-Piccolo Antibody used in Immunohistochemistry (IHC) on Mouse Muscle (SPC-197) Rabbit Anti-Piccolo Antibody used in Western blot (WB) on Mouse, Rat brain cell lysates (SPC-197)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.