HSP110 Antibody | StressMarq Biosciences SPC-195D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP110 Antibody

catalog :

SPC-195D

quantity :

100 µg

price :

251.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1f, s2a, 2c, 6c	Inda M, Joshi S, Wang T, Bolaender A, Gandu S, Koren Iii J, et al. The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction. Nat Commun. 2020;11:319 pubmed publisher
western blot; human; loading ...; fig 4b	Rodina A, Wang T, Yan P, Gomes E, Dunphy M, Pillarsetty N, et al. The epichaperome is an integrated chaperome network that facilitates tumour survival. Nature. 2016;538:397-401 pubmed publisher
	Nitika -, Truman A. Endogenous epitope tagging of heat shock protein 70 isoform Hsc70 using CRISPR/Cas9. Cell Stress Chaperones. 2018;23:347-355 pubmed publisher
	Hashikawa N, Utaka Y, Ogawa T, Tanoue R, Morita Y, Yamamoto S, et al. HSP105 prevents depression-like behavior by increasing hippocampal brain-derived neurotrophic factor levels in mice. Sci Adv. 2017;3:e1603014 pubmed publisher
	Tunnah L, MacKellar S, Barnett D, MacCormack T, Stehfest K, Morash A, et al. Physiological responses to hypersalinity correspond to nursery ground usage in two inshore shark species (Mustelus antarcticus and Galeorhinus galeus). J Exp Biol. 2016;219:2028-38 pubmed publisher

image

image 1 :

Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

product information

Catalog No :

SPC-195D

Product Name :

HSP110 Antibody

Description :

Rabbit Anti-Hamster HSP110 Polyclonal

Target :

HSP110

Conjugate :

Unconjugated

2021 List Price :

251.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins

Alternative Name(s) :

Heat shock 105kDa alpha Antibody, heat shock 105kDa beta Antibody, HSP105 Antibody, HSP105A Antibody, HSP105B Antibody, HSP110 Antibody, HSPH1 Antibody, NY CO 25 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse

Immunogen Species :

Hamster

Accession Number :

NP_006635.3

Swiss-Prot :

Q92599

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Mk Hm Sh Ys Shk

Species Reactivity Full Name :

Human Mouse Rat Bovine Monkey Hamster Sheep Yeast Shark Gummy Shark (Mustelus antarcticus) School Shark (Galeorhinus galeus)

Antibody Dilution :

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~110kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-195D, RRID: AB_2119373

Certificate of Analysis :

1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm

Scientific Background :

HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

References :

1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733. 2. Dragovic Z., et al. (2006) EMBO J. 25: 2519-2528. 3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859. 4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-195_HSP110_Antibody_WB_Human_Cell-line-lysates_1.png
SPC-195_HSP110_Antibody_IHC_Mouse_backs
kin_1.png

Image Alternative Text :

Rabbit Anti-HSP110 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-195) Rabbit Anti-HSP110 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-195)

more info or order :

StressMarq Biosciences product webpage