product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
HSPB2 (MKBP) Antibody
catalog :
SPC-194D
quantity :
100 µg
price :
300.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of Rat tissue mix showing detection of HSPB2 protein using Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSPB2 Polyclonal Antibody (SPC-194) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
product information
Catalog No :
SPC-194D
Product Name :
HSPB2 (MKBP) Antibody
Description :
Rabbit Anti-Human HSPB2 (MKBP) Polyclonal
Target :
HSPB2
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins
Alternative Name(s) :
DMPK-binding protein Antibody, heat shock 27kDa protein 2 Antibody, HSP27 Antibody, HSPB2 Antibody, MKBP Antibody, MGC133245 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Synthetic peptide
Immunogen Species :
Human
Accession Number :
NP_001532.1
Swiss-Prot :
Q16082
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS, 50% glycerol, 0.02% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~27kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-194D, RRID: AB_2120948
Certificate of Analysis :
1 µg/ml of SPC-194 was sufficient for detection of MKBP in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSPs, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP's localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
References :
1. Pinz I., et al. (2008) FASEB J. 22: 84-92.
2. Suzuki A., et al. (1998) J Cell Bio. 140(5): 1113-1124.
3. Sugiyama Y., et al. (2000) J Biol Chem. 275: 1095-1104.
4. Oshita S.E., et al. (2010) Breast Cancer Res Treat. Epub: 20087649.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-194_HSPB2_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-194_HSPB2_Antibody_WB_Rat_tissue-mix_1.png
Image Alternative Text :
Rabbit Anti-HSPB2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-194) Rabbit Anti-HSPB2 Antibody used in Western blot (WB) on Rat tissue mix (SPC-194)
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
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