product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Cpn10 Antibody
catalog :
SPC-193D
quantity :
100 µg
price :
220.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-193D image 1
Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
product information
Catalog No :
SPC-193D
Product Name :
Cpn10 Antibody
Description :
Rabbit Anti-Human Cpn10 Polyclonal
Target :
Cpn10
Conjugate :
Unconjugated
2021 List Price :
220.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Metabolism Mitochondrial Metabolism Mitochondrial Markers
Alternative Name(s) :
10kDa Chaperonin Antibody, Chaperonin 10 Antibody, Cpn 10 Antibody, EPF Antibody, GROES Antibody, Heat Shock 10kD protein1 Antibody, HSP10 Antibody, HSPE1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Human Cpn10 peptide AA 91-101
Immunogen Species :
Human
Accession Number :
NP_002148.1
Swiss-Prot :
P61604
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Rt Bv Dg GP Pg Rb Sh Xe
Species Reactivity Full Name :
Human Mouse Rat Bovine Dog Guinea Pig (Cavia porcellus) Pig Rabbit Sheep African clawed frog (Xenopus laevis)
Antibody Dilution :
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS pH7.3, 0.02% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~ 10kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-193D, RRID: AB_2119080
Certificate of Analysis :
1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Mitochondrion Mitochondrion Matrix
Scientific Background :
Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
References :
1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-193_Cpn10_Antibody_IHC_Mouse_backskin_1.png SPC-193_Cpn10_Antibody_WB_Rat_brain-cell-lysates_1.png
Image Alternative Text :
Rabbit Anti-Cpn10 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-193) Rabbit Anti-Cpn10 Antibody used in Western blot (WB) on Rat brain cell lysates (SPC-193)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.