product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
HSP40 Antibody
catalog :
SPC-184D
quantity :
100 µg
price :
300.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
Plasmodium falciparum 3D7
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-184D image 1
Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000.
image 2 :
StressMarq Biosciences SPC-184D image 2
Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000.
product information
Catalog No :
SPC-184D
Product Name :
HSP40 Antibody
Description :
Rabbit Anti-P. Falciparum HSP40 Polyclonal
Target :
HSP40
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins
Alternative Name(s) :
DNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
C-terminal peptide of Pf11_0513 conjugated to KLH
Immunogen Species :
P. falciparum
Accession Number :
XP_001348169.3
Swiss-Prot :
Q8IHM7
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
P.fa
Species Reactivity Full Name :
Plasmodium falciparum
Antibody Dilution :
WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1.8 mg/ml
Specificity :
Detects ~ 62kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-184D, RRID: AB_2094595
Certificate of Analysis :
0.9 µg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
References :
1. Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240. 2. Melville M.W., et al. (1997) PNAS USA. 94: 97-102. 3. Hattori H., Let al. (1992) Cell Structure and Function. 17: 77-86. 4. Ohtsuka K. Masuda A., Nakai A., and Nagata K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 5. Bardwell J.C.A., et al. (1986) J. Biol. Chem. 261: 1782-1785. 6. Ohku M., Tamura F., Nishimura S., and Uchida H. (1986) J. Biol. Chem. 261: 1778-1781.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-184_HSP40_Antibody_WB_Parasite_lysa
tes_1.png
Image Alternative Text :
Rabbit Anti-HSP40 Antibody used in Western blot (WB) on Parasite lysates (SPC-184)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.